Equal amounts of protein (30 μg) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to activated/pre-wetted PVDF membranes. The membranes were hybridized with the following primary antibodies as indicated: AT8 anti-P-tau pSer202/Thr205 (1:500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1:1000, Abcam, #ab109390); anti-total tau T46 (1:1000, Thermo Scientific, #13-6400); anti-GAPDH (1:2000, Santa Cruz, #sc-32233); anti=P-CaMKII (1:1000, Abcam, #ab32678); anti-PSD-95 (1:1000, Millipore, #7E3-1B8), C1q (1:1000, Abcam, #ab182451). Secondary antibodies included: peroxidase labeled anti-mouse IgG (1:2000, Vector Laboratories); peroxidase labeled anti-rabbit IgG (1:2000, Vector Laboratories); peroxidase labeled anti-rat IgG (1/2000, Vector Laboratories). ECL (Pierce®) was used to reveal the immunoreactive proteins, and images were acquired using a Fujifilm ImageReader LAS-4000. Membranes were stripped using a stripping buffer (Thermo Scientific) when required. Luminescent immunoreactive protein bands were quantified using Fiji software (ImageJ).
Peroxidase labeled anti mouse igg
Manufactured by Vector Laboratories
Peroxidase-labeled anti-mouse IgG is a secondary antibody conjugate used for the detection of mouse immunoglobulin G (IgG) in various immunoassays and immunohistochemical techniques. It consists of an anti-mouse IgG antibody labeled with the enzyme horseradish peroxidase, which can catalyze a colorimetric reaction for signal amplification and visualization.
Automatically generated - may contain errors
Lab products found in correlation
Bis tris gel, by Bio-Rad (3 mentions)
Image reader las 4000, by Fujifilm (3 mentions)
Stripping buffer, by Thermo Fisher Scientific (3 mentions)
Anti gapdh, by Santa Cruz Biotechnology (3 mentions)
Peroxidase labeled anti rabbit igg, by Vector Laboratories (3 mentions)
Peroxidase labeled anti rat igg, by Vector Laboratories (3 mentions)
Anti psd95, by Merck Group (2 mentions)
Anti p camkii, by Abcam (2 mentions)
At8 anti p tau pser202 thr205, by Thermo Fisher Scientific (2 mentions)
Anti p tau ps396, by Abcam (2 mentions)
Anti total tau t46, by Thermo Fisher Scientific (2 mentions)
Ecl western blotting detection reagent, by GE Healthcare (1 mentions)
4 protocols using peroxidase labeled anti mouse igg
1
Quantitative Protein Analysis in Tau Pathology
2
Western Blot Analysis of Tau and Synaptic Proteins
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Equal amounts of protein (30 μg) were separated by electrophoresis in precast 4–12% Bis-Tris Gels (Bio-Rad) and transferred to activated/pre-wetted polyvinylidene difluoride membranes. The membranes were hybridized with the following primary antibodies as indicated: AT8 anti-P-tau pSer202/Thr205 (1:500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1:1000, Abcam, #ab109390); anti-total tau T46 (1:1000, Thermo Scientific, #13-6400); anti-GAPDH (1:2000, Santa Cruz, #sc-32233); anti-P-CaMKII (1:1000, Abcam, #ab32678); anti-PSD-95 (1:1000, Millipore, #7E3-1B8), C1q (1:1000, Abcam, #ab182451). Secondary antibodies included: peroxidase-labeled anti-mouse IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rabbit IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rat IgG (1:2000, Vector Laboratories). ECL (Pierce®) was used to reveal the immunoreactive proteins, and images were acquired using a Fujifilm ImageReader LAS-4000. Membranes were stripped using a stripping buffer (Thermo Scientific) when required. Luminescent immunoreactive protein bands were quantified using Fiji software (ImageJ).
3
Detecting Ace2 Protein by Western Blot
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Monoclonal anti-Ace2 antibody (AF933; R&D Systems, Minneapolis, MN) was used to detect the Ace2 protein in cell lysates. Peroxidase-labeled anti-mouse IgG (Vector Laboratories, Burlingame, CA) was used as the secondary antibody. Blots were detected using ECL Western blotting detection reagents (GE Healthcare, Buckinghamshire, United Kingdom).
4
Western Blot Analysis of Tau Phosphorylation
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Equal amounts of protein from (30 μg, mouse cortex was used) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to nitrocellulose membranes. The membranes were hybridized with the following primary antibodies as indicated: TYROBP (1:500, antibody provided in collaboration with Dr Richard W. Cho at Cell Signaling Technology); AT8 anti-pTAU pSer202/Thr205 (1:1000, Thermo Scientific, #MN1020); anti-GAPDH (1:2000, Santa Cruz, #SC-32233), anti-p-TAU Thr205 (1:1000, Invitrogen, #44-738G), anti-TAU HT7 (1:1000, Invitrogen, #MN1000). Secondary antibodies included: peroxidase-labeled anti-mouse IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rabbit IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rat IgG (1:2000, Vector Laboratories). ECL (Pierce, #32106) was used to reveal the immunoreactive proteins, and images were acquired using a Fujifilm ImageReader LAS-4000. Membranes were stripped using a stripping buffer (Thermo Scientific, #46430) when required. Luminescent immunoreactive protein bands were quantified using Fiji software (ImageJ).
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