Cd95 clone dx2

Manufactured by BioLegend

Sourced in United States

CD95 (clone DX2) is a lab equipment product from BioLegend. It is a monoclonal antibody that binds to the CD95 antigen.

Automatically generated - may contain errors

Lab products found in correlation

Lsr 2 flow cytometer, by BD (1 mentions) Cd3 clone sp34 2, by BD (1 mentions) Hla dr clone l243, by BD (1 mentions) Pd 1 clone eh12.2h7, by BioLegend (1 mentions) Foxp3 clone pch101, by Thermo Fisher Scientific (1 mentions) Cd4 clone l200, by BD (1 mentions) Ki67 clone b56, by BD (1 mentions) Cd28 clone cd28, by Beckman Coulter (1 mentions) Cd8 clone 3b5, by Thermo Fisher Scientific (1 mentions) Cd127 clone hil 7r m21, by BD (1 mentions) Cd3 clone okt3, by BioLegend (1 mentions) Cd27 clone m t271, by BioLegend (1 mentions) Macsquant 16 flow cytometer, by Miltenyi Biotec (1 mentions) Igm clone mhm 88, by BioLegend (1 mentions) Cd14 clone m5e2, by BioLegend (1 mentions)

Spelling variants of this product

CD95-BV605 (clone DX2) (3 citations)

2 protocols using cd95 clone dx2

Multiparameter Phenotypic Characterization of PBMCs

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Thawed PBMCs were surface stained with Aqua amine reactive viability dye (Invitrogen) and mAbs to CD45 (clone D058–1283, BD Biosciences), CD3 (clone SP34–2, BD Biosciences), CD4 (clone L200, BD Biosciences), CD8 (clone 3B5, Invitrogen), CD95 (clone DX2, BioLegend), CD28 (clone CD28.2, Beckman), CD127 (clone hIL-7R-M21, BD Biosciences), PD-1 (clone EH12.2H7, BioLegend), and HLA-DR (clone L243, BD Biosciences) for 20 min at room temperature, fixed, and then permeabilized with FoxP3 Fix/Perm Buffers (eBioscience) per the manufacturer’s instructions. Permeabilized cells were then stained intracellularly for Ki67 (clone B56, BD Biosciences) and FoxP3 (clone PCH101, eBioscience) for 30 min at 4°C, washed in FoxP3 Perm/Wash buffer, fixed in 0.5% paraformaldehyde and analyzed by flow cytometry on a BD LSRII flow cytometer (BD Biosciences) and analyzed using Flowjo Software (Tree Star Inc).

Swainson L.A., Ahn H., Pajanirassa P., Khetarpal V., Deleage C., Estes J.D., Hunt P.W., Munoz-Sanjuan I, & McCune J.M. (2019). Kynurenine 3-monooxygenase inhibition during acute SIV infection lowers PD-1 expression and improves post-cART CD4+ T cell counts and body weight: KMO inhibition improves clinical outcome in SIV infection. Journal of immunology (Baltimore, Md. : 1950), 203(4), 899-910.

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Characterizing B cell subsets by flow cytometry

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After preparation of freshly isolated PBMCs in flow cytometry buffer, PBMCs were stained with fluorescent labeled antibodies and measured on a MACSQuant 16 flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). B cell subsets were stained using antibodies against CD19 (clone REA675, Miltenyi), CD20 (clone REA780, Miltenyi), CD27 (clone M-T271, Biolegend, San Diego, California, US), CD138 (clone BB4/MI15, Biolegend), HLA-DR (clone REA332, Miltenyi), IgD (clone REA740, Miltenyi), IgM (clone MHM-88, Biolegend), IgA (clone M24A, Merck Millipore), CD95 (clone DX2, Biolegend) as well as CD3 (clone OKT3, Biolegend) and CD14 (clone M5E2, Biolegend) for the dump channel. Definition of B cell populations: naive B cells: CD19⁺CD20⁺CD27^-; memory B cells: CD19⁺CD20⁺CD27⁺; Plasmablasts and plasma cells: CD19⁺CD20^-CD27^hi).

Bernardes J.P., Mishra N., Tran F., Bahmer T., Best L., Blase J.I., Bordoni D., Franzenburg J., Geisen U., Josephs-Spaulding J., Köhler P., Künstner A., Rosati E., Aschenbrenner A.C., Bacher P., Baran N., Boysen T., Brandt B., Bruse N., Dörr J., Dräger A., Elke G., Ellinghaus D., Fischer J., Forster M., Franke A., Franzenburg S., Frey N., Friedrichs A., Fuß J., Glück A., Hamm J., Hinrichsen F., Hoeppner M.P., Imm S., Junker R., Kaiser S., Kan Y.H., Knoll R., Lange C., Laue G., Lier C., Lindner M., Marinos G., Markewitz R., Nattermann J., Noth R., Pickkers P., Rabe K.F., Renz A., Röcken C., Rupp J., Schaffarzyk A., Scheffold A., Schulte-Schrepping J., Schunk D., Skowasch D., Ulas T., Wandinger K.P., Wittig M., Zimmermann J., Busch H., Hoyer B.F., Kaleta C., Heyckendorf J., Kox M., Rybniker J., Schreiber S., Schultze J.L, & Rosenstiel P. (2020). Longitudinal Multi-omics Analyses Identify Responses of Megakaryocytes, Erythroid Cells, and Plasmablasts as Hallmarks of Severe COVID-19. Immunity, 53(6), 1296-1314.e9.

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