AECII (2.5 × 105 cells/mL) were seeded into a 96-well plate. Then cells were maintained in DMEM/F12 with or without TM in the incubator at 5% CO2 and 37 °C. The plate was removed on days 0.5, 1, 2, 3, and 4 for the MTT assay. Briefly, 10µL MTT solution (5 g/L) (Solarbio) was added to each well. Cells were then further incubated at 37 °C for 24 h. This was followed by the addition of 100 µl DMSO. The plate was then slightly shaken and mixed evenly for 10 min. An automatic enzyme-labeled reading meter (Thermo) was used to measure the optical density value at 490 nm.
Automatic enzyme labeled reading meter
Manufactured by Thermo Fisher Scientific
The Automatic Enzyme-Labeled Reading Meter is a device designed for the quantitative analysis of enzyme-labeled samples. It measures the optical density of the samples, providing accurate and reliable results. The core function of this instrument is to facilitate automated detection and measurement of enzyme-labeled substances, without making any interpretations or extrapolations about its intended use.
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Lab products found in correlation
2 protocols using automatic enzyme labeled reading meter
1
MTT Assay for AECII Viability
2
Evaluating Tunicamycin's Effect on LR-MSC
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LR-MSC (2.5 × 105 cells/mL) were seeded into a 96-well plate. Then, cells were maintained in DMEM with or without tunicamycin in the incubator at 5% CO2 and 37 °C. The plate was removed on 24 h and 48 h for the MTT assay. Briefly, 10µL MTT solution (5 g/L) (Solarbio, Beijing, China) was added to each well. Cells were then further incubated at 37 °C for 24 h. This was followed by the addition of 100μL DMSO. The plate was then slightly shaken and mixed evenly for 10 min. An automatic enzyme-labeled reading meter (Thermo) was used to measure the optical density value at 490 nm.
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