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3 protocols using 4 methylpentan 2 ol

1

Volatile Compounds in Food Analysis

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The following compounds (food grade, purity in parenthesis) were purchased from Sigma-Aldrich (St. Louis, MO, USA): ethyl acetate (≥99%), ethanol (96%), hexanal (≥97%), 4-methylpentan-2-ol (98%), E-2-hexenal (≥95%), hexan-1-ol (≥98%), E-3-hexen-1-ol (97%), E,E-2,4-hexadienal (95%), nonanal (≥98%), acetic acid (≥99.5%), E-2-decenal, pentanoic acid (≥99%), hexanoic acid (≥98%), and E,E-2,4-decadienal (≥89%).
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2

Analysis of Volatile Organic Compounds

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Chemical standards of acetone, butane-2,3-dione, butan-2-one, thiophene, dimethyl disulfide, hexanal, heptan-4-one, heptan-2-one, 2-pentylfuran, dimethyl trisulfide, 6-methylhept-5-en-2-one, benzaldehyde, octanal, d-limonene, trans-3-octen-2-one, linalool, 4-methylphenol, 1,3-di-tert-butylbenzene, internal standard (4-methylpentan-2-ol), and sodium chloride (NaCl, ≥99.5%) were supplied by Sigma-Aldrich (Saint Louis, MO, USA). MilliQ water (Millipore, Bedford, MO, USA) was used for the preparation of standards. Hydrochloric acid (HCl, 37%) was purchased from Chempur (Piekary Śląskie, Poland). The 75-μm carboxen/polydimethylsiloxane (CAR/PDMS) (stable flex) solid-phase microextraction (SPME) fibers were purchased from Supelco (Bellefonte, PA, USA).
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3

Analytical Standards for Phytochemical Analysis

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Authentic standard of rutin and Folin-Ciocalteu reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). All the used solvents were of HPLC grade purity (BDH Laboratory Supplies, Poole, UK). 4-methylpentan-2-ol used as internal standard and a mixture of linear alkanes (C9–C30) in hexane used for calculating linear retention indexes were from Sigma-Aldrich (St. Louis, MO, USA). Inert gasses (He and N2 99.999% purity) were supplied by SOL gas company (Monza, Italy).
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