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Anti β actin c4

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Anti-β-actin (C4) is a mouse monoclonal antibody that recognizes the β-actin protein, a widely expressed cytoskeletal protein. This antibody is commonly used as a loading control in Western blot analysis to normalize protein expression levels across samples.

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Lab products found in correlation

Western lighting plus ecl system, by PerkinElmer (1 mentions) Anti gata 3 d13c9, by Cell Signaling Technology (1 mentions) Anti histone h3, by BioLegend (1 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Bradford assay, by Bio-Rad (1 mentions) Cdx2 88, by BioGenex (1 mentions) Anti ng2, by Merck Group (1 mentions) Anti mag, by Santa Cruz Biotechnology (1 mentions) Anti olig2, by Merck Group (1 mentions) Anti jagged1, by Santa Cruz Biotechnology (1 mentions) Anti gfap, by Merck Group (1 mentions) Anti hes1, by Merck Group (1 mentions) Anti iba1, by Fujifilm (1 mentions) Anti cnpase, by Fortrea (1 mentions) Anti mbp, by Fortrea (1 mentions) Anti gfp, by Abcam (1 mentions) Anti cd31, by BD (1 mentions)

3 protocols using anti β actin c4

1

Western Blot for Nuclear and Cytoplasmic Proteins

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Cells were lysed in RIPA buffer, and whole-cell extracts were quantified by the Bradford assay (Bio-Rad). For assessment of nuclear proteins, nuclear extracts were obtained using NE-PER nuclear and cytoplasmic extraction kit (Thermo Scientific). The protein samples or cell lysates were analyzed by SDS-PAGE and Western blot. Briefly, after proteins were transferred onto PVDF membranes (Millipore), the membranes were incubated with indicated primary antibodies, followed by a HRP-conjugated secondary antibody. Immunoreactive bands were detected using the Western Lighting Plus-ECL system (PerkinElmer) or the SuperSignal West Dura Extended Duration Substrate (Pierce). The primary antibodies used for Western blot included anti-p38 (2F11, Millipore), anti-phospho p38 (Thr180/Tyr182) (2BB10, Cell Signaling), and anti-β-actin (C4, Millipore), anti-NKG2A (Abnova), anti-GATA-3 (D13C9, Cell Signaling), anti-phospho-GATA-3 (Ser308) (EPR18118, Abcam), and anti-Histone H3 (BioLegend).
Chang M.C., Cheng H.I., Hsu K., Hsu Y.N., Kao C.W., Chang Y.F., Lim K.H, & Chen C.G. (2019). NKG2A Down-Regulation by Dasatinib Enhances Natural Killer Cytotoxicity and Accelerates Effective Treatment Responses in Patients With Chronic Myeloid Leukemia. Frontiers in Immunology, 9, 3152.
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2

Western Blot Analysis of CDX2 and β-Actin

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Whole bone marrow or AGK463 protein extracts separated on SDS/PAGE were analyzed by western blot using mouse monoclonal anti‐CDX2 (CDX2‐88, BioGenex, Fremont, CA, USA; dilution 1/2000) and mouse monoclonal anti‐β‐actin (C4, Millipore, Dachstein, France; dilution 1/25 000) antibodies. HRP‐conjugated secondary anti‐mouse antibody was used for ECL detection (GE Company, Amersham, UK).
Galland A., Gourain V., Habbas K., Güler Y., Martin E., Ebel C., Tavian M., Vallat L., Chenard M., Mauvieux L., Freund J., Duluc I, & Domon‐Dell C. (2021). CDX2 expression in the hematopoietic lineage promotes leukemogenesis via TGFβ inhibition. Molecular Oncology, 15(9), 2318-2329.
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3

Immunohistochemical Profiling of CNS Markers

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For immunohistochemical, immunocytochemical, and Western blot procedures please reference the Supplemental Experimental Procedures. Antibodies used for immunohistochemistry were anti-GFP (Abcam, 1:500), anti-Brdu (Accurate,1:200, 30 min 2N HCl followed by 15 min 0.1M Boric Acid brain section pre-treatment), anti-NG2 (Millipore, 1:500), anti-GFAP (Sigma, 1:500), anti-ET-1 (Abbiotec, 1:200), anti-CD31 (BD Biosciences, 1:500), anti-Jagged-1 (Iowa Hybridoma Bank, 1:200), anti-IBA1 (Wako, 1:500), anti-MAG (Santa Cruz, 1:200), anti-MBP (Covance (SMI-99p), 1:1000), anti-Hes1 (Millipore, 1:1000), anti-CD11b/MAC1 (ABD Serotec, 1:400), anti-Olig2 (Millipore, 1:500), and anti-APC (Ab-7) (CC-1) (Calbiochem, 1:500). Antibodies used for immunocytochemistry were anti-GFP (Abcam, 1:500), anti-O1 (R&D systems, 1:500), anti-GFAP (Sigma, 1:500), and anti-NG2 (Millipore, 1:500). Antibodies used for Western Blot analysis include anti-MBP (Covance (SMI-99p), 1:5000), anti-MAG (Santa Cruz (sc-15324), 1:200), anti-CNPase (Covance, 1:500), anti-Jagged1 (Santa Cruz (sc-135955), 1:200), anti-β-actin(C4) (Millipore, 1:5000), anti-GFAP (Sigma, 1:5000), and anti-NICD (Iowa Hybridoma Bank C17.9C6, 1:1000).
Hammond T.R., Gadea A., Dupree J., Kerninon C., Nait-Oumesmar B., Aguirre A, & Gallo V. (2014). Astrocyte-derived Endothelin-1 inhibits remyelination through Notch activation. Neuron, 81(3), 588-602.
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