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2 protocols using p dimethylaminebenzaldehyde

1

Analytical Techniques for Phytochemical Profiling

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Solvents including hexane (95%), chloroform (HPLC grade), methanol (HLPC grade), and acetonitrile (HPLC grade) were obtained from LAB-SCAN (Gliwice, Poland) and absolute ethanol, sodium carbonate (Na2CO3), and sulfuric acid (H2SO4) from Panreac (Barcelona, Spain). Potassium hydroxide (KOH), ascorbic acid, 2,6-Di-tert-butyl-p-cresol (BHT), bovine serum albumin (BSA), acetylacetone, p-dimethylaminebenzaldehyde, HCl (37%), phenol, hexadecane, ergosterol (95%), D-glucose, D-glucosamine hydrochloride, FAME (fatty acid methyl ester), and gallic acid were purchased from Sigma–Aldrich (Madrid, Spain) as well as acarbose and α-glucosidase from Saccharomyces Cerevisae and α-amylase from the porcine pancreas. All reagents and solvents were of analytical grade.
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2

Comprehensive Chemical Analysis Protocol

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Hexane (95%), HPLC-grade chloroform, mEthanol and acetronitrile solvents were obtained from LAB-SCAN (Gliwice, Poland). Ethanol (100%), sulfuric acid (H2SO4), and sodium carbonate (Na2CO3) were obtained from Panreac (Barcelona, Spain). Ascorbic acid, ergosterol (95%), D-glucose, gallic acid, and D-glucosamine hydrochloride standards were purchased from Sigma-Aldrich (Madrid, Spain). Potassium hydroxide (KOH), 2,6-Di-tert-butyl-p-cresol (BHT), hexadecane, bovine serum albumin (BSA), p-dimethylaminebenzaldehyde, HCl (37%), acetylacetone, phenol sulfuric were also from Sigma-Aldrich. All reagents and solvents used were of analytical grade.
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