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4 protocols using chloroauric acid haucl4

1

Graphene Oxide-Supported Catalysts Synthesis

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Graphene oxide (GO, thickness, 0.8–1.2 nm) was purchased from Nanjing XFNANO Materials Tech. Co., Ltd and employed as the support. 1,10-Phenanthroline(C12H8N2, 97.0%), Zeolite (Permutit) and γ-Al2O3 were all purchased from Aladdin. Carbon black VX 72 was bought from Cabot Corporation. Acetone, Cobalt(II) chloride hexahydrate (CoCl2·6H2O, 99.0%), Copper(II) chloride dihydrate (CuCl2·2H2O, 99.0%), Manganese(II) chloride tetrahydrate (MnCl2·4H2O, 99.0%), and Palladium(II) chloride (PdCl2, 99.99%) were received from Sinopharm Chemical Reagent Co., Ltd. Rhodium(III) chloride trihydrate (RhCl3·3H2O, 98%), Chloroauric acid (HAuCl4), and Chloroplatinic acid hexahydrate (H2PtCl6·6H2O, 99.99%) were purchased from Shanghai Macklin Biochemical Co., Ltd. Nickel chloride hexahydrate (NiCl2·6H2O, 99.999%), Stannic chloride hydrated (SnCl4·5H2O, 99.995%), and Iridium chloride (IrCl3, 99.8%) were received from Aladdin. All the reagents were used without further purification. Ultrapure Milli-Q water (18.2 MΩ cm−1) was used to prepare all the aqueous solutions and wash samples. Ethanol was received from Shanghai Lingfeng chemical regent Co., Ltd. Pt/C (20 wt%) was purchased from MACKLIN Biochemical Technology Co., Ltd.
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2

Synthesis of Ti3AlC2-Supported Au Nanoparticles

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Ti3AlC2 powder (98% purity, 400 mesh) was purchased from Liaoning 11 Sci-Tech Co., LTD. (Changchun, China). Chloroauric acid (HAuCl4) was purchased from Macklin (Shanghai, China). Sodium hydroxide (≥96%, NaOH) and urea (99%) were purchased from Sinopharm Chemical Reagent Company Co., Ltd. (Shanghai, China). Benzyl alcohol (99%), benzaldehyde (99%), benzoic acid (99%), toluene (99.8%), and sodium borohydride (NaBH4, 98%) were purchased from Shanghai Titan Technology Co., LTD. (Shanghai, China). Cinnamic alcohol (98%) was purchased from Shanghai Xushuo biotechnology Co., Ltd. (Shanghai, China). 4-Methylbenzyl alcohol (98%), 4-methoxylbenzyl alcohol (98%), and poly (diallyldimethylammonium chloride) solution (PDDA, 20 wt% in water) were purchased from Aladdin Industrial Inc., Shanghai, China.
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3

Synthesis and Characterization of Au NPs

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The following materials were used as purchased: nylon membrane (0.1 mm pore diameter, Shanghai Xingya purification material factory); glass dish (10 mm diameter, Shanghai JingAn Biological Science and Technology Ltd).; chloroauric acid (HAuCl4, Macklin) sodium citrate tribasic dihydrate (Na3C6H5O7·2H2O, 99%, Macklin); p-aminophenol (4-ATP, 97%, Macklin); thiram (99.2%, Beijing Zhongjian Navigation Mark Metering Technology Co. Ltd); phorate (100 μg mL−1, 95.9%, Beijing Zhongjian Navigation Mark Metering Technology Co. Ltd); benthiocarb (98.1%, Dr Ehrenstorfer GmbH). The water was obtained from a Milli-Q system. Au NPs were synthesized via the sodium citrate reduction method, which was modified by Frens et al.20 (link) (details in the ESI).
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4

Microplates-based Immunoassay Protocol

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Microplates
were purchased
from JET. The ultrafiltration tube (50 k) was purchased from Bioground
(Chongqing). HBSAg, HBcAg, HBeAg, enzyme-labeled antibody diluent,
GAbs, and MAbs were purchased from Bai Aotong (Luoyang). 2-(4-Morpholino)ethanesulfonic
acid (MES), catalase, and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) were purchased
from Sigma. NH2–PEG–COOH was purchased from
Peng Sheng Biological (Shanghai). The PSs–COOH (300 nm) was
made in the laboratory. Chloroauric acid (HAuCl4) was purchased
from Macklin. BSA was purchased from BioFroxx. The remaining drugs
were bought from Chron Chemicals. All the experiments used ultrapure
water (18.2 MΩ cm).
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