Recombinant trimeric spike proteins (5 μg/mL) were plated in 50 μl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 μl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 μl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 μl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 μl of 1-Step TMB Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 μl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
Hrp goat anti mouse
Manufactured by BioLegend
HRP goat anti-mouse is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse primary antibodies in various immunoassays and immunochemical procedures.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using hrp goat anti mouse
1
SARS-CoV-2 Spike Protein ELISA
2
ELISA for Measuring Mouse RBD Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RBD (5 µg/mL) was plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 µl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 µl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 µl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 µl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
3
ELISA for Measuring Mouse RBD Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RBD (5 µg/mL) was plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 µl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 µl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 µl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 µl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
4
ELISA for Measuring Mouse RBD Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RBD (5 µg/mL) was plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature. These were washed 3 times with 300 µl of ddH2O using an ELx 405 Bio-Tex plate washer and blocked with 150 µl of ChonBlock (Chondrex) for at least 1 h at room temperature. Plates were washed 3x with 300 µl of 1x PBST. Mouse serum samples, serially diluted in diluent buffer (1x PBS, 0.1% Tween) starting at 1:50 serum dilution was then added to coated plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate at room temperature for 1 h and then washed 6x with PBST. Finally, the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well, and the plates were quenched with 50 µl of 2 M H2SO4 to each well. Plates were read at 450 nm and normalized for path length using a BioTek Synergy HT Microplate Reader.
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