Mouse tgf β1 duo set

To measure IgA levels in colon tissues, feces and mucus were flushed and homogenized with endotoxin-free PBS. The supernatants were harvested after centrifugation (800G, 15 min) and stored at −80°C for later analysis. The cell supernatants of sort-purified B cells that underwent in vitro culture were also collected for cytokine and antibody measurements. The concentrations of IgA, IL-10, IL-6, and IgM were measured using the Mouse Ready-SET-Go! ELISA kits (eBioscience) according to the manufacturer’s instructions. The levels of active TGF-β were measured using a human TGF-β1 duo-set (DY240) or mouse TGF-β1 duo-set (DY1679) with a substrate reagent pack (DY999) according to the manufacturer’s instructions (R&D Systems Europe, Abingdon, UK) (25 (link), 26 (link)).