Peripheral blood mononuclear cells (PBMCs) were isolated from patients with SLE and controls according to the manufacturer’s protocol (Ficoll‐Paque PLUS; GE Healthcare). The cells were added to a tissue culture plate precoated with anti‐CD3 antibody (10 μg/ml) (eBioscience) together with soluble anti‐CD28 antibody (1 μg/ml) (eBioscience), interleukin 6 (IL‐6) (10 ng/ml), interleukin 1β (IL‐1β) (10 ng/ml), interleukin 2 (10 ng/ml), transforming growth factor β1 (TGF‐β1) (10 ng/ml) (Immuno Tools), and interleukin 23 (IL‐23) (10 ng/ml) (R&D Systems). Cells were kept in culture medium for 6 days to polarize Th17 and Treg cells, with phorbol myristate acetate (50 ng/ml) and ionomycin (1 μg/ml) (Sigma Aldrich) stimulation for 5 hours and with or without anti‐CD95 antibody (1 μg/m) (BD Bioscience) stimulation for 3 hours on the day of harvest. Atorvastatin (5 μmol/l) (Sigma Aldrich) was added 1 day before harvest for the following experiments.
Interleukin 1β il 1β
Manufactured by Immunotools
Sourced in Germany
Interleukin 1β (IL-1β) is a pro-inflammatory cytokine that plays a central role in the regulation of immune and inflammatory responses. It is produced by various cell types, including monocytes, macrophages, and dendritic cells, in response to various stimuli such as microbial products, IL-1, or damage to tissue. IL-1β is involved in the activation of the immune system and the inflammatory response, and it has a wide range of biological effects.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 antibody, by Thermo Fisher Scientific (1 mentions)
Soluble anti cd28 antibody, by Thermo Fisher Scientific (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Ficoll paque plus, by GE Healthcare (1 mentions)
Atorvastatin, by Merck Group (1 mentions)
Interleukin 6 il 6, by Thermo Fisher Scientific (1 mentions)
Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions)
Interleukin 2 il 2, by Immunotools (1 mentions)
2 protocols using interleukin 1β il 1β
1
PBMC Isolation and Th17/Treg Modulation
2
Cytokine-Induced Killer Cell Generation
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Cytokine induced killer cells were generated in vitro from human PBMC according to the standard protocol developed by Schmidt-Wolf et al. in 1991 [6 (link)]. In short, non-adherent Ficoll-separated (Lymphoprep, PAA) human PBMC were cultured in RPMI-1640 medium containing 10% heat-inactivated FCS, 25 mmol/L Hepes (PAA), 1% P/S. Next, (5 × 106) cells/mL were seeded out. On Day 0, 1000 U·mL−1 interferon gammy (IFN-γ) (ImmunoTools, Friesoythe, Germany) was added to generate CIK cells. Then, 300 U/mL interleukin-2 (IL-2), 100 U/mL interleukin-1β (IL-1β) (both ImmunoTools) and 50 ng/mL anti-CD3 (α-CD3) (eBioscience, Frankfurt, Germany) were added after 24 h. Every three days some medium was exchanged and 300 U/mL IL-2 was added again. After two weeks CIK cells were mature and ready to use. The cells were incubated at 37 °C in humidified 5% CO2 atmosphere.
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