Anti-β-Catenin (catalog no. 610153) and anti-CD133 antibody (catalog no. 566598) was purchased from BD Bioscience. Anti-PGK1 (catalog no. 68540 S), anti-EEA1 (catalog no. 3288 S), anti-β-actin (catalog no. 4970), anti-H3 (catalog no. 4499) and anti-H3K4me3 (catalog no. 9751 S) antibodies were from Cell Signaling Technology. Anti-ZIC2 (catalog no. ARP35821_P050) antibody was purchased from Aviva Systems Biology. Anti-TOM40 (catalog no. 18409-1-AP), anti-TOM70 (catalog no. 14528-1-AP), anti-c-MYC (catalog no. 10828-1-AP) and anti-AXIN2 (catalog no. 20540-1-AP) antibodies were from Proteintech Group, Inc. Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 antibody (catalog no. A-11005), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (catalog no. A-11008), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (catalog no. A-21244) were purchased from Invitrogen. HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) antibody (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) antibody (catalog no. SA00001-2) were purchased from Proteintech Group, Inc. Polymer HRP and AP detection kits were from Beyotime Biotechnology. Biotin labeled RNA mix (catalog no. 11685597910) was from Roche.
Anti pgk1
Manufactured by Cell Signaling Technology
Anti-PGK1 is a primary antibody product offered by Cell Signaling Technology. It is designed to detect the Phosphoglycerate Kinase 1 (PGK1) protein, which is an essential enzyme involved in the glycolysis pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti h3, by Cell Signaling Technology (1 mentions)
Anti β catenin, by BD (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti h3k4me3, by Cell Signaling Technology (1 mentions)
Anti eea1, by Cell Signaling Technology (1 mentions)
Anti tom70, by Proteintech (1 mentions)
Anti cd133 antibody, by BD (1 mentions)
Anti tom40, by Proteintech (1 mentions)
Anti c myc, by Proteintech (1 mentions)
Proteinase inhibitor, by Merck Group (1 mentions)
Ripa reagent, by Merck Group (1 mentions)
Ecl detection system, by Bio-Rad (1 mentions)
Anti kiaa1429, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti pgk1
1
Antibody and Detection Reagent Purchasing
2
Protein Isolation and Western Blot Analysis
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Cells with the indicated treatment were subjected to protein isolation by RIPA reagents supplemented with 1% proteinase inhibitor (Sigma, USA) and quantified by BCA Protein Assay Kit (Thermo Fisher Scientific). Proteins (∼30 μg) were electrophoresed to 12% SDS-PAGE gels and then transferred to PVDF membranes. Membranes were blocked in BSA (5%) for 2 h and then incubated with primary antibodies (anti-KIAA1429, Cell Signaling Technology, #88,358, 1:1000; anti-PGK1, Cell Signaling Technology, #63,536, 1:1000) overnight at 4 °C. Then, proteins were carefully incubated with secondary antibody for 1 h. Membranes were washed with TBST solution three times. Lastly, signals were detected by ECL detection system (Bio-Rad, California, USA).
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