Fluorescent droplets were incubated in plastic Cell Counter slides (Bio-Rad) at room temperature. Chambers were sealed using nail varnish to prevent evaporation during aging. Fluorescence recovery after bleaching was monitored using Zen software on a Zeiss LSM 510 Meta NLO confocal microscope. For intradroplet FRAP, a circular are of 1μM radius was bleached in droplets with a radius between 5μM and 10μM. Raw data was background substracted and normalized using Excell, and plotted using Prism software. FRAP curves were fitted with a one phase exponential curve. Images were formatted FIJI and ImageJ software.
Cell counter slides
Manufactured by Bio-Rad
Sourced in United States
The Cell Counter slides are designed for use with Bio-Rad's cell counting instruments. They provide a standardized way to prepare and measure cell samples, enabling accurate and consistent cell counting.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 510 meta nlo confocal microscope, by Zeiss (2 mentions)
Trypan blue dye, by Bio-Rad (1 mentions)
Silybin, by Merck Group (1 mentions)
Silychristin, by Merck Group (1 mentions)
Silydianin, by Merck Group (1 mentions)
Jc 1 dye, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Histopaque 1077, by Merck Group (1 mentions)
3 protocols using cell counter slides
1
Fluorescent Droplet FRAP Analysis
2
Fluorescent FUS LC Droplet Dynamics
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FUS LC droplets were generated by diluting the stock solution to 250μM in 50μM MES buffer at pH 5, 200μM NaCl. 1μM of Alexa 488 tagged FUS LC was spiked into the solution.
Fluorescent droplets were incubated in plastic Cell Counter slides (Bio-Rad) at room temperature. Chambers were sealed using nail varnish to prevent evaporation during aging. Every two hours three arbitrary fields were imaged on a Zeiss LSM 510 Meta NLO confocal microscope. Droplet sizes were quantified with FIJI and analyzed with Prism.
Fluorescent droplets were incubated in plastic Cell Counter slides (Bio-Rad) at room temperature. Chambers were sealed using nail varnish to prevent evaporation during aging. Every two hours three arbitrary fields were imaged on a Zeiss LSM 510 Meta NLO confocal microscope. Droplet sizes were quantified with FIJI and analyzed with Prism.
3
Flavonolignans Cytotoxicity and Oxidative Stress
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Dimethyl sulfoxide (DMSO), 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), glucose, Tris, NADH, sodium pyruvate, Histopaque®-1077, RPMI 1640 and DMEM mediums as well as the flavonolignans (silybin, silychristin and silydianin), were all obtained from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Trypan blue dye and cell counter slides were sourced from Bio-Rad (Hercules, California, USA). Both the JC-1 Dye and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA Dye), came from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals were reagent grade or the highest-quality available.
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