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Detailed Immunostaining and Inhibitor Protocol

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Antibodies against FN (Cat#15613-1-AP), COL1A1 (Cat#67288-1-lg), α-SMA (Cat#55135-1-AP, Cat#67735-1-AP), FGFR1 (Cat#60325-1-lg), CD31 (Cat#60287-1-lg), CD45 (Cat#11265-1-lg), EpCAM (Cat#60287-1-lg), Vimentin (Cat#10366-1-AP) and GAPDH (Cat#60004-1-lg) were purchased from Proteintech (Rosemont, IL, USA). Antibodies against p-EGFR (Cat#3777S), EGFR (Cat#4267), p-VEGFR2 (Cat#2478), VEGFR2 (Cat#9698), PDGFRβ (Cat#3169), p-PLCγ1 (Cat#2821), PLCγ1 (Cat#5690), p-GAB1 (Cat#12745), GAB1 (Cat#3232), p-SHC (Cat#2434), SHC (Cat#2432), p-AKT (Cat#4060), AKT (Cat#9272), p-ERK1/2 (Cat#4370), ERK1/2 (Cat#4695), p-STAT3 (Cat#4113), STAT3 (Cat#9139), GRB2 (Cat#3972), GST (Cat#2642) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against pY (Cat#EPR16871), p-PDGFRβ (Cat#ab248657), p-FGFR1 (Cat#ab59194), α-SMA (Cat#ab124964), pS/pT (Cat#ab117253) and IgG (Cat#EPR25A and Cat#ab37415) were purchased from Abcam (Cambridge, MA, USA). Bleomycin, nintedanib and PHPS1 were purchased from Targetmol (Boston, MA, USA).
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2

Antibody Profiling for Cell Analysis

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Antibodies against Fibronectin, E-cadherin, α-SMA, Dach1, Fsp-1, Zo-1, Epcam and β-actin were purchased from ProteinTech. Antibodies against B-cell lymphoma 2 protein (Bcl-2), Bax, cleaved caspase-3, caspase-3, Bim, Foxo1, and C-Jun were obtained from Cell Signaling Technology. Anti-Pro-Spc antibody was purchased from Millipore (Germany). Anti-Collagen I antibody was obtained from Servicebio (China). Anti-Dach1 antibody for immunoprecipitation and anti-CD68 antibody were purchased from Santa Cruz Biotechnology.
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