Ammonium thiocyanate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Ammonium thiocyanate is a chemical compound used as a laboratory reagent. It is a colorless, crystalline solid that is soluble in water. Ammonium thiocyanate is commonly used in various analytical and testing procedures in scientific research and industrial applications.

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4 protocols using ammonium thiocyanate

Chemical Reagents for Analytical Testing

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The chemicals used in the present work were purchased from various companies. Sodium nitrite, potassium persulfate, dipotassium hydrogenphosphate, ferric chloride, monosodium dihydrogenphosphate, potassium ferricyanide, trichloroacetic acid, disodium hydrogenphosphate, Rutin, Mueller-Hinton agar (MHA), and Folin-Ciocalteu reagent were purchased from Merck (Darmstadt, Germany), gallic acid from Riedel-de-Haen (Seelze, Germany), ammonium molybdate, linoleic acid, ferrous chloride tetrahydrate and aluminum chloride from BDH Labs (Cambridge, England), DPPH (2,2-diphenyl-1-picrylhydrazyl) and ascorbic acid from MP Biomedicals (Illkirch, France), sodium acetate from Daejung (Siheung City, Korea), ammonium thiocyanate from Alfa-Aesar (Karlsruhe, Germany), amoxicillin from GlaxoSmithKline (Karachi, Pakistan) and trichloroacetic acid, TPTZ (2,4,6 tripyridyl-s-triazine), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were purchased from Sigma-Aldrich (Steinheim, Germany).

Ahmed D., Khan M.M, & Saeed R. (2015). Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves. Antioxidants, 4(2), 394-409.

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Cabazitaxel Solubility Enhancement

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The following materials were obtained: Cabazitaxel (Carbosynth Cat. # FC19621); deuterated cabazitaxel (1 mg reference standard; Toronto Research Chemicals Cat. # C046502–1MG); Pluronic F127 (Sigma Cat. # P2443); clotrimazole (Alfa Aesar Cat. # J63895), ketoconazole (Alfa Aesar Cat. # J63367), cobalt nitrate hexahydrate (Alfa Aesar Cat. # 36418), ammonium thiocyanate (Alfa Aesar Cat. # A10632), Tween-80 / Polysorbate 80 (VWR Cat. # EM-9490), nimodipine (TCI Cat. # N0896), and mifepristone (TCI Cat. # M1732). The following solvents were used: ethyl acetate (Fisher), acetone (Fisher), methylene chloride (Fisher), acetonitrile (Fisher), and ethanol (Decon).

Sun B., Huang J., Mabrouk M.T., Zhang Y., Jin H, & Lovell J.F. (2020). A surfactant-stripped cabazitaxel micelle formulation optimized with accelerated storage stability. Pharmaceutical development and technology, 25(10), 1281-1288.

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Camelina Oil Extraction and Protein Purification

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Certified organic camelina seed was purchased from a local market in Seattle, WA, USA, and its oil was extracted by a screw press (AgoilPress, M70 Oil Press Eau Clair, Wisconsin, USA). Whey protein isolate (WPI) was obtained from a local super market in Lincoln, NE, USA. Ammonium thiocyanate and barium chloride were purchased from Fisher scientific (Fair Lawn, NJ, USA). Ferrous sulfate heptahydrate was obtained from MP Biochemicals, LLC (Solon, OH, USA). All other reagents and solvents were of analytical or chromatographic grade and purchased from Sigma Aldrich (St. Louis, MO, USA).

Belayneh H.D., Wehling R.L., Zhang Y, & Ciftci O.N. (2017). Development of omega‐3‐rich Camelina sativa seed oil emulsions. Food Science & Nutrition, 6(2), 440-449.

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Liposome Synthesis and Characterization

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Peptides of >95% purity were synthesized by BioSynthesis, Inc. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (POPG), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (PEG2k-PE), 1,2-dimyristoleoyl-sn-glycero-3-phosphocholine (diC14:1PC), 1,2-dieicosenoyl-sn-glycero-3-phosphocholine (C20:1PC), phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhodamine-PE) and Cholesterol were purchased from Avanti Polar Lipids. Labeled low density lipoprotein (BODIPY™ FL LDL), Cholera Toxin Subunit B (Recombinant) with Alexa Fluor™ 488 Conjugate (Labeled CTX), 10 kDa dextran with Alexa Fluor™ 488 Conjugate, 8-Aminonaphthalene-1,2,3-trisulfonic acid (ANTS) and p-xylylenebis (pyridinium bromide) (DPX) were purchased from Thermo Fisher Scientific. Doxorubicin HCl was purchased from Cayman Chemical Company. Chloroform, ammonium thiocyanate, and other salts and buffer materials were purchased from Fisher Scientific or Sigma-Aldrich. TAMRA-biotin-dextran (TBD) was synthesized as described elsewhere.^{13 (link)}

Sun L., Hristova K, & Wimley W.C. (2021). Membrane-selective Nanoscale Pores in Liposomes by a Synthetically Evolved Peptide: Implications for Triggered Release. Nanoscale, 13(28), 12185-12197.

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