To detect changes in physiological indices under salt and drought stress conditions, leaves were collected from plants at different time points (0, 10, 17, 21, and 28 d) during stress treatments. Electrolytic leakage, the contents of chlorophyll, MDA, and H2O2, and the enzyme activities of SOD, CAT, and POD were measured. For extraction of H2O2, CAT, SOD, and POD, 0.2 g of leaves were sampled with 1.8 ml phosphate-buffered saline (PBS; 0.1 mol/L, pH 7.4) on ice. The crude extract was centrifuged at 10,000 g for 10 min at 4 °C. The H2O2 content, and CAT, SOD, and POD enzyme activities in the supernatant were immediately measured by enzyme-linked immunosorbent assay (ELISA), using the corresponding detection kits (A064-1, A007-1, A001-1, and A084-3; Jiancheng, China). MDA content analysis was performed using the thiobarbituric acid method [53 (link)]. Chlorophyll was extracted and examined using the protocol [54 (link)]. Electrolyte leakage was determined by relative conductivity as described by Hu [55 (link)].
A064 1
Manufactured by Nanjing Jiancheng
Sourced in China
The A064-1 is a laboratory equipment designed for scientific research and analysis. It is a versatile instrument capable of performing various tasks. The core function of the A064-1 is to provide accurate and reliable measurement and analysis capabilities for researchers and scientists in their work.
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2 protocols using a064 1
1
Analyzing Plant Stress Responses
2
Mulberry Seed Germination and Antioxidant Assays
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Mulberry seeds germination began after 2 days of treatment (seeds were considered germinated when radicles appeared), and germinating seeds were observed and counted every 48 hours to calculate the germination percentage. After 14 days, mulberry seedlings from different treatments were collected and rinsed with deionized water. The germination rate and radicle length of mulberry seedlings were determined, and the final germination rate was the number of seeds germinated divided by the total number of seeds used for the test × 100.
In this experiment, SOD, POD, CAT activities as well as H 2 O 2 content and MDA content were detected using biological assay kits A001-1-1, A084-3, A007-1, A064-1 and A003-1-1 respectively (Nanjing Jiancheng Bioengineering Institute, Nanjing,China) according to the manufacturer's instructions. SOD activity was determined by measuring the absorbance at 550 nm using the xanthine oxidase method. In addition, the POD activity was determined by gauging the absorbance at 420 nm using guaiacol and H 2 O 2 as substrates, and CAT activity was determined by measuring the absorbance at 405 nm using H 2 O 2 as a substrate. The malondialdehyde content was determined through a thiobarbituric acid reactive substance (TBARS) assay. Three biological replicates were detected.
In this experiment, SOD, POD, CAT activities as well as H 2 O 2 content and MDA content were detected using biological assay kits A001-1-1, A084-3, A007-1, A064-1 and A003-1-1 respectively (Nanjing Jiancheng Bioengineering Institute, Nanjing,China) according to the manufacturer's instructions. SOD activity was determined by measuring the absorbance at 550 nm using the xanthine oxidase method. In addition, the POD activity was determined by gauging the absorbance at 420 nm using guaiacol and H 2 O 2 as substrates, and CAT activity was determined by measuring the absorbance at 405 nm using H 2 O 2 as a substrate. The malondialdehyde content was determined through a thiobarbituric acid reactive substance (TBARS) assay. Three biological replicates were detected.
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