Madin-Darby canine kidney (MDCK) cells (ATCC® CCL-34TM) were grown in minimal essential medium (MEM; 61100-61, Gibco, United States) supplemented with 10% fetal bovine serum (FBS; 10082-147, Gibco, United States), penicillin G sodium 100 units/ml, streptomycin sulfate 100 μg/ml, and amphotericin B 250 ng/ml (antibiotic-antimycotic; 15240-062, Gibco, United States). Human renal cell line HEK293T cells (ATCC® CRL-3216TM) were grown in Dulbecco’s modified Eagle medium (DMEM; 12100-046, Gibco, United States) supplemented with 10% FBS and antibiotics as described above.
Ccl 34tm
Manufactured by American Type Culture Collection
Sourced in United States
The CCL-34TM is a laboratory equipment product provided by American Type Culture Collection. It is a cell line that serves as a core function in biological research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Hep 2 cells, by American Type Culture Collection (1 mentions)
Ccl 23tm, by American Type Culture Collection (1 mentions)
2 7 dichlorofluorescin diacetate dcf, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Vitamin c, by Merck Group (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Na2seo3, by Merck Group (1 mentions)
3 protocols using ccl 34tm
1
Cell Culture Protocol for MDCK and HEK293T
2
Comparative Efficacy of Influenza and RSV Growth
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Madin-Darby Canine Kidney (MDCK) cells (ATCC number, CCL-34TM) and HEp-2 cells (ATCC number, CCL-23TM) were purchased from ATCC (Manassas, VA 20110, USA.) and used for the propagation and initial detection of viruses based on cytopathic effect. Eagle Minimal Essential Medium (EMEM) (Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, USA) were used to grow the MDCK and HEp-2 cell lines. These were used in combination to observe the comparative efficacy for the growth of both influenza and RSV. Method of Ken et al.11 was followed to set up of assay.
3
MDCK Cells Influenza Virus Assay
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The Madin–Darby Canine Kidney (MDCK) cells were obtained from ATCC® CCL-34TM. H1N1 influenza virus was provided by the Virus Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University. Fetal bovine serum and DMEM were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Na2SeO3, vitamin C, AM, MTT, propidium iodide, 4′,6-diamidino-2-phenylindole (DAPI), and 2′,7′-dichlorofluorescin (DCF)-diacetate were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). AKT and caspase-3 antibodies were purchased from Cell Signaling Technology (Boston, MA, USA).
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