Amyloid β42 mouse elisa kit

Tissues of the cortex were collected as described above. Concentration of Aβ42 in mouse brains was detected using an Amyloid β42 Mouse ELISA kit (KMB3441, Thermo Fisher Scientific, Inc.) according to the manufacturer’s protocol. Aβ42 content was examined by extraction of homogenate pellets in 5 M guanidine-HCl. Briefly, equal amounts of protein in each group were diluted to 100 μl volume and incubated in plate wells at room temperature for 2 h. The plate wells were then washed and incubated with 100 μl of Detection Antibody solution for 1 h at room temperature. Afterwards, the wells were washed and incubated with 100 μl of HRP-linked antibody solution for 30 min followed by four times of wash. Finally, the optical density was read at 450 nm on a spectrophotometer (Bio-Rad) after the adding of Stop Solution.

AcH3K18 ELISA was performed using EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Colorimetric) (EpiGentek). CSF samples were concentrated by 10-fold using Amicon Ultra Centrifugal Filter Unit with Ultracel-3K membrane (Millipore). Mouse β-tubulin ELISA was performed using Mouse TUB β ELISA kit (MyBiosource MBS723313), according to manufacturer’s protocol. Mouse Aβ40 and Aβ42 ELISA was performed using Amyloid β 40 and Amyloid β 42 Mouse ELISA Kit (Thermo Fisher), according to manufacturer’s protocol. Sensitive Tau ELISAs were adapted and modified according to previous reports [24 (link), 25 (link)] and as described previously [41 (link)]. Briefly, mouse monoclonal antibody BT2 or HT7 was used for capture. The respective analytes were detected with alkaline phosphatase–conjugated mouse monoclonal antibody against Tau5 (BioLegend). Recombinant full length hTau (rPeptide) was used to generated standard curves for each assay. The CDP-Star substrate (Invitrogen) was used as chemiluminescent alkaline phosphatase substrate.