Alpha-modified eagle medium (α-MEM) and fetal bovine serum (FBS) were purchased from Gibco-BRL (Sigma–Aldrich, St. Louis, MO, USA). PIN was purchased from CSNpharm (Catalog No. CSN15662, Shanghai, P.C. China) and dissolved in dimethylsulfoxide (DMSO). Tartrate-resistant acid phosphatase (TRAcP) staining kit were purchased from Sigma–Aldrich (St. Louis, MO, USA). Macrophage colony-stimulating factor (M-CSF) was provided by R&D Systems (Minneapolis, MN, USA). Recombinant murine sRANK-ligand protein (rm-sRANKL) was obtained from PeproTech (Rocky Hill, NJ, USA).
Tartrate resistant acid phosphatase tracp staining kit
Manufactured by Merck Group
Sourced in United States, Japan
The Tartrate-resistant acid phosphatase (TRAcP) staining kit is a laboratory tool used to detect and visualize the presence of the TRAcP enzyme. TRAcP is a biomarker associated with certain cell types and is commonly used in research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Macrophage colony stimulating factor m csf, by R&D Systems (1 mentions)
Alpha minimum essential medium α mem, by Thermo Fisher Scientific (1 mentions)
Phospho p38, by Abcam (1 mentions)
Phospho erk, by Abcam (1 mentions)
Phospho jnk, by Abcam (1 mentions)
Recombinant mouse m csf, by R&D Systems (1 mentions)
C fos, by Abcam (1 mentions)
β actin, by Abcam (1 mentions)
2 protocols using tartrate resistant acid phosphatase tracp staining kit
1
Osteoclastogenesis Induction Protocol
2
Osteoclast Differentiation Assay
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DIO with a purity > 97% was purchased from Mansite (Chengdu, China) and prepared at a stock concentration of 100 mM in dimethylsulfoxide (DMSO). Alpha minimum essential medium (α-MEM) and fetal bovine serum (FBS) were obtained from ThermoFisher (Sydney, Australia). Recombinant mouse M-CSF was obtained from R&D Systems (Minneapolis, MN, USA). Recombinant GST-rRANKL was prepared as previously described (Xu et al., 2000) .
Antibodies against ERK, phospho-ERK, p38, phospho-p38, JNK, phospho-JNK, IκBα, c-Fos and β-actin were obtained from Abcam (Cambridge, MA, USA). Antibody specific for NFATc1 was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The cell viability kit (CCK-8) was obtained from Dojindo Molecular Technologies (Kumamoto, Japan).
Tartrate-resistant acid phosphatase (TRAcP) staining kit was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Antibodies against ERK, phospho-ERK, p38, phospho-p38, JNK, phospho-JNK, IκBα, c-Fos and β-actin were obtained from Abcam (Cambridge, MA, USA). Antibody specific for NFATc1 was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The cell viability kit (CCK-8) was obtained from Dojindo Molecular Technologies (Kumamoto, Japan).
Tartrate-resistant acid phosphatase (TRAcP) staining kit was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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