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Mirna 132

Manufactured by Thermo Fisher Scientific
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MiRNA-132 is a laboratory instrument designed for the detection and analysis of microRNA-132, a small non-coding RNA molecule involved in gene expression regulation. The core function of this product is to provide researchers with a tool to measure and quantify the levels of miRNA-132 in biological samples.

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Lab products found in correlation

Opti mem 1, by Thermo Fisher Scientific (2 mentions) Control mirna, by Thermo Fisher Scientific (2 mentions) Siport neofx transfection reagent, by Thermo Fisher Scientific (2 mentions) Label it mirna labeling kit, by Mirus Bio (2 mentions) Cy 3 labelled control mirna, by Thermo Fisher Scientific (2 mentions) αmirna 132, by Thermo Fisher Scientific (2 mentions)

2 protocols using mirna 132

1

miRNA-132 Transfection in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Cy-3-labelled Control miRNA was purchased from Life Technologies. miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies) were labelled using Label IT miRNA Labeling Kit (Mirus) according to manufacturer’s protocol. Cells were transfected with Control miRNA (Life Technologies), miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies). The miRNAs were transfected with siPORT NeoFX transfection reagent (Life Technologies) at a concentration of 50 nM and 1 × Opti-MEM I (Life Technologies). All transfections were completed according to the manufacturer’s protocols for 24 h.
Connor Y., Tekleab S., Nandakumar S., Walls C., Tekleab Y., Husain A., Gadish O., Sabbisetti V., Kaushik S., Sehrawat S., Kulkarni A., Dvorak H., Zetter B., R. Edelman E, & Sengupta S. (2015). Physical nanoscale conduit-mediated communication between tumour cells and the endothelium modulates endothelial phenotype. Nature Communications, 6, 8671.
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2

miRNA-132 Transfection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Cy-3-labelled Control miRNA was purchased from Life Technologies. miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies) were labelled using Label IT miRNA Labeling Kit (Mirus) according to manufacturer's protocol. Cells were transfected with Control miRNA (Life Technologies), miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies). The miRNAs were transfected with siPORT NeoFX transfection reagent (Life Technologies) at a concentration of 50 nM and 1 × Opti-MEM I (Life Technologies). All transfections were completed according to the manufacturer's protocols for 24 h.
Connor Y., Tekleab S., Nandakumar S., Walls C., Tekleab Y., Husain A., Gadish O., Sabbisetti V., Kaushik S., Sehrawat S., Kulkarni A., Dvorak H., Zetter B., R. Edelman E, & Sengupta S. (2015). Physical nanoscale conduit-mediated communication between tumour cells and the endothelium modulates endothelial phenotype. Nature Communications, 6, 8671.
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