PPa was obtained from Shanghai Xianhui Pharmaceutical Co., Ltd. DSPE-PEG2K was purchased from A.V.T. (Shanghai) Pharmaceutical Co., Ltd. GA was obtained from Dalian Meilun Biotech Co., Ltd., China. 2’,7’-dichlorofluorescin diacetate (DCFH-DA) was purchased from Genview. GSH assay kit was obtained from Nanjing Jiancheng Bioengineering Institute. Hoechst 33342 was purchased from BD Biosciences, USA. Mouse TXN ELISA Kit was purchased by Shanghai COIBO Biotechnology Co., Ltd. Cell culture dishes and plates were bought from NEST Biotechnology Co., Ltd (Wuxi, China). Other solvents were of analytical or high performance liquid chromatography (HPLC) grade.
Cell culture dishes and plates
Manufactured by NEST Biotechnology
Sourced in China
Cell culture dishes and plates are laboratory equipment used for the cultivation and growth of cells in a controlled environment. They provide a sterile, enclosed space for the cells to adhere and proliferate. These dishes and plates come in various sizes and designs to accommodate different cell types and experimental needs.
Automatically generated - may contain errors
5 protocols using cell culture dishes and plates
1
Nanoparticle-Based Antioxidant Delivery
2
Synthesis and Characterization of Lipid-Based Nanomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DTX and DiR were sourced from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). 1-hydroxybenzotriazole (HOBt), 1-(3-Dimethylaminopropyl)−3-ethylcarbodiimide hydrochloride (EDCI) and 4-dimethylaminopyrideine (DMAP) were sourced from Energy Chemical (Shanghai, China). Dithiodiglycolic acid was bought from TCL(Shanghai) Development Co., Ltd (Shanghai, China). SA, OA, EA, LA, LNA and coumarin-6 (C-6) were obtained from Aladdin (Shanghai, China). Dithiothreitol (DTT), cell culture media and MTT were bought from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). DSPE-MPEG2000 was bought from A. V. T. (Shanghai, China) Pharmaceutical Technology Co., Ltd (Shanghai, China). Cell culture dishes and plates were bought from NEST Biotechnology Co., Ltd (Wuxi, China).
3
Profiling Human Liver Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following human liver cancer cell lines were used: (a) SMCC7721 and Hep3B cells were kindly provided by Dr. Zhiyong Mao (Tongji University), (b) HCCLM9 cells were obtained from the Wuhan University School of Medicine, (c) HepG2, HepG2.2.15, BEL7402, Huh7, FHCC98, and the immortalized non-malignant human normal liver cell line L02 were stored in our lab and (e) the stably transfected cell lines L02-C1orf61, BEL7402-C1orf61, HCCLM9 sh-C1orf61, Huh7 sh-C1orf61 were established by our lab. HCCLM9 C1orf61-/-cell lines were established in our lab using the CRISPR/Cas system. Cells were all cultured in DMEM supplemented with 10% fetal bovine serum, penicillin (1000 U/ml), and streptomycin (1000 µg/ml) in a humidi ed chamber with 5% CO 2 . Cell culture dishes and plates were obtained from Wuxi NEST Biotechnology Co. Ltd. (Wuxi, China).
4
Comprehensive Characterization of Nanoparticle-Mediated Thrombin Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DiR, coumarin-6 (C6), and thrombin from bovine plasma (37KD) were obtained from Meilun Biotech Co. Ltd., (Dalian, China). BPA, and Sodium nitrite (NaNO2) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Griess Reagent was obtained from was purchased from Beijing Solarbio Science & Technology Co., ltd. 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-PEG2K) was purchased from A.V.T. Pharmaceutical Co., Ltd (Shanghai, China). DSPE-PEG2K-CREKA was synthesized by Premierbiochem Co., Ltd (Suzhou, China). sCD40L Assay Kit was purchased from Beijing Solarbio Science & Technology Co., ltd. Cell culture dishes and plates were bought from NEST Biotechnology Co., Ltd (Wuxi, China). All solvents and reagents used were analytical or HPLC standard.
5
Culturing Diverse Hematopoietic Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HL60 cells were purchased from CCTCC (China Center for Type Culture Collection; Wuhan, China). K562, U937 and THP-1 cells were kindly provided by Dr. Zan Huang (Wuhan University). All cell lines were grown in RPMI 1640 medium supplemented with 10% FBS (fetal bovine serum, Hyclone), 1% streptomycin and 1% penicillin. AML patient cells were provided by Dengju Li (Tongji Hospital of Tongji Medical College; Wuhan, China). The informed consent was obtained from all of the examined subjects, and the related studies were approved by the ethics committees of the participating hospitals and institute. AML patient cells were cultured in expansion media (RPMI 1640 with 10% FBS, 10 ng/ml recombinant human IL-3, 10 ng/ml rhIL-6, and 50 ng/ml stem cell factor). All cells were cultured in a humidified atmosphere containing 5% CO2 at 37 °C. Cell culture dishes and plates were purchased from Wuxi NEST Biotechnology Co. Ltd. (Wuxi, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!