Mc car

Manufactured by American Type Culture Collection

Sourced in United States

The MC/CAR is a laboratory equipment product designed for cell culture applications. It functions as a controlled-atmosphere chamber, providing a regulated environment for culturing cells. The core purpose of the MC/CAR is to maintain specific temperature, humidity, and gas composition settings to support optimal cell growth and development.

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Lab products found in correlation

Rpmi8226, by American Type Culture Collection (2 mentions) Mm 1r, by American Type Culture Collection (2 mentions) Ez pcr mycoplasma detection kit, by Sartorius (2 mentions) Mm 1s, by American Type Culture Collection (1 mentions) Fetal calf serum (fcs), by Lonza (1 mentions) Hsb 2, by American Type Culture Collection (1 mentions) Oci my5, by American Type Culture Collection (1 mentions) Anbl 6, by American Type Culture Collection (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Kms11, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Busulfan, by Merck Group (1 mentions) Venetoclax, by Selleck Chemicals (1 mentions) Gemcitabine, by Selleck Chemicals (1 mentions) Ampflstr identifier kit, by Thermo Fisher Scientific (1 mentions) Panobinostat, by Selleck Chemicals (1 mentions) Melphalan, by Merck Group (1 mentions) Rpmi 1640, by Selleck Chemicals (1 mentions)

4 protocols using mc car

MC/CAR Cell Culture Protocol

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The Human leukemia cell line MC/CAR was purchased from ATCC (USA). Recommended media RPMI1640 supplemented with 10% fetal bovine serum was used as the culture medium. The cell culture volume was enlarged every 2 days and harvested for inoculation 8 days after resuscitation.

Wang Y., Zheng C., Zhuang C., Fu Q., Qin J., Zhang B., Bian Y., Qi N, & Zhu J. (2021). Preclinical pharmacology and toxicology evaluation of an anti-CD52 monoclonal antibody produced by perfusion fermentation process. Journal of Industrial Microbiology & Biotechnology, 48(9-10), kuab078.

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Preparation of Multiple Myeloma Cell Lysates

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MM cell lines U266, McCAR, HSB2, MM1S, RPMI8226, OPM2, H929, ANBL6, OCIMY5, AMO1, and KMS11 were obtained from ATCC (Manassas, VA). The T2 cell line, a human B- and T-cell hybrid expressing HLA-A2 molecules, was provided by Dr J. Molldrem (University of Texas M. D. Anderson Cancer Center, Houston, TX). The cell lines were cultured in DMEM media supplemented with 10% fetal calf serum (BioWhittaker, Walkersville, MD), 100 IU/ml penicillin, and 100 µg/ml streptomycin (Gibco-Life Technologies, Rockville, MD). A mixture of ten MM cell lines was utilized to prepare tumor cell lysates by repeated (10X) cycles of freeze (−140 °C)/thaw (37 °C) or prepared as irradiated (20 Gy) whole tumor cells as sources of MM antigen stimulation.

Bae J., Accardi F., Hideshima T., Tai Y.T., Prabhala R., Shambley A., Wen K., Rowell S., Richardson P.G., Munshi N.C, & Anderson K.C. (2021). Targeting LAG3/GAL-3 to overcome immunosuppression and enhance anti-tumor immune responses in multiple myeloma. Leukemia, 36(1), 138-154.

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Characterization of Leukemia and Lymphoma Cell Lines

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Cell lines used in this study included acute myeloid leukemia (AML), lymphoma and multiple myeloma. KBM3/Bu250⁶ or KBU, is an alkylating-agent resistant human AML cell line developed in our laboratory [23 (link)]; OCI-AML3 and MOLM13 AML cell lines were obtained from the laboratory of Dr. Michael Andreeff (UT MD Anderson Cancer Center, Houston, TX, USA). The lymphoma cell lines J45.01, Toledo, U937 and the multiple myeloma cell lines RPMI 8226, MM.1R and MC/CAR were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in RPMI 1640 medium (Mediatech, Manassas, VA, USA) supplemented with 10% heat-inactivated fetal bovine serum (GeminiBio, Sacramento, CA, USA) and 100 U/mL penicillin and 100 μg/mL streptomycin (Mediatech) at 37°C in a fully humidified atmosphere of 5% CO₂ in air. Absence of mycoplasma contamination was confirmed using the EZ-PCR mycoplasma detection kit (Biological Industries, Cromwell, CT, USA).

Dulay R.M., Valdez B.C., Li Y., Chakrabarti S., Dhillon B., Kalaw S.P., Reyes R.G, & Cabrera E.C. (2021). Cytotoxicity of Gymnopilus purpureosquamulosus extracts on hematologic malignant cells through activation of the SAPK/JNK signaling pathway. PLoS ONE, 16(5), e0252541.

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Myeloma Cell Line Cultivation and Validation

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MM.1R, MC/CAR and U266B1 cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). RPMI 8226 vr10 was a kind gift from Dr. R. Orlowski (UT MD Anderson Cancer Center, Houston, TX, USA). Cells were cultured in RPMI 1640 (Mediatech, Manassas, VA, USA) supplemented with 15% heat-inactivated fetal bovine serum (Atlanta Biologicals, Inc., Flowery Branch, GA, USA) and 100 U/ml penicillin and 100 μg/ml streptomycin (Mediatech) at 37°C in a fully humidified atmosphere of 5% CO₂ in air. Cells were validated by short tandem repeat DNA fingerprinting using the Amp-FlSTR Identifier kit according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). Mycoplasma contamination was determined using the EZ-PCR mycoplasma detection kit (Biological Industries, Cromwell, CT, USA). Busulfan and melphalan (Sigma-Aldrich, St Louis, MO, USA) were dissolved in dimethyl sulfoxide (DMSO); gemcitabine, panobinostat and venetoclax (SelleckChem, Houston, TX, USA) were dissolved in DMSO and diluted in RPMI 1640 prior to use.

Valdez B.C., Li Y., Murray D., Liu Y., Nieto Y., Bashir Q., Qazilbash M.H, & Andersson B.S. (2020). Panobinostat and venetoclax enhance the cytotoxicity of gemcitabine, busulfan, and melphalan in multiple myeloma cells. Experimental hematology, 81, 32-41.

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