16‐week‐old chow diet‐fed mice and HFD‐fed mice were acclimated into home cage of Promethion Core System (Sable Systems International, Beijing, China) for 24 h before the start of the experimental record. VO2 and VCO2 level were measured for 1 min in a 9 min interval for 4 consecutive days and locomotor activity was measured continuously by breaks of light beams. O2 consumed (VO2), CO2 produced (VCO2), and energy expenditure (EE) were calculated following the manufacture's manual, and the respiratory exchange ratio (RER) was computed by the ratio of VCO2/VO2. Finally, EE was analyzed either after the normalization by LBM or locomotor activity using covariance (ANCOVA).
Promethion core system
Manufactured by Sable Systems
Sourced in China
The Promethion Core System is a modular laboratory platform designed for precise and versatile gas exchange measurements. It features a highly sensitive and reliable open-circuit calorimetry system that can accurately quantify oxygen consumption, carbon dioxide production, and respiratory quotient. The core system provides a stable and controlled environment for conducting a wide range of physiological and metabolic studies.
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Lab products found in correlation
3 protocols using promethion core system
1
Metabolic Profiling of Chow and HFD Mice
2
Metabolic Profiling of Chow and HFD Mice
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Sixteen weeks old Chow and HFD mice were acclimated into home cage of Promethion Core System (Sable Systems International) for 24 h before the start of the experimental record. Then, O 2 and CO 2 exchange were continuously recorded every 9 min for 1 min for 4 days, and locomotor activity was continuously recorded by interruptions of infrared light beams. O 2 consumed (VO 2 ), CO 2 produced (VCO 2 ), and energy expenditure (EE) were calculated following the manufacture's manual, and the respiratory exchange ratio (RER) was computed by the ratio of VCO 2 /VO 2 . Finally, EE was analyzed either after the normalization by LBM or locomotor activity using covariance (ANCOVA).
3
Metabolic Profiling of Chow and HFD Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sixteen weeks old Chow and HFD mice were acclimated into home cage of Promethion Core System (Sable Systems International) for 24 h before the start of the experimental record. Then, O 2 and CO 2 exchange were continuously recorded every 9 min for 1 min for 4 days, and locomotor activity was continuously recorded by interruptions of infrared light beams. O 2 consumed (VO 2 ), CO 2 produced (VCO 2 ), and energy expenditure (EE) were calculated following the manufacture's manual, and the respiratory exchange ratio (RER) was computed by the ratio of VCO 2 /VO 2 . Finally, EE was analyzed either after the normalization by LBM or locomotor activity using covariance (ANCOVA).
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