Thermo hplc ms system

HPLC-MS analysis was performed on the Thermo HPLC-MS system (Thermo Scientific, Waltham, MA, USA) using the Thermo Hypersil GOLD C₁₈ column (1.9-μm particle size, 2.1 mm × 100 mm) with methanol and water as eluents. The experimental conditions were following: injection volume: 5 μM; mobile phase: 0–0.2 min, 95% B; 0.2–3.5 min, 95%–2% B; 3.5–5 min, 2% B; 5–7.5 min, 2%–95% B; 7.5–10 min, 95% B; flow rate: 0.3 ml·min^–1. The HPLC eluate was administered to the MS system with a spray voltage of 1.0 kV. The MS peaks were recorded and compared with that of the FX standard.

HPLC-MS analysis was performed on the Thermo HPLC-MS system (Thermo Scientific, Waltham, MA, USA) using the Thermo Hypersil GOLD C18 column (1.9-μm particle size, 2.1 × 100 mm) with acetonitrile (A) and water (B) as eluents. The experimental conditions were as following: injection volume: 7 μM; mobile phase: 0–1 min, 100% B; 1–4 min, 100%–5% B; 4–9.5 min, 5% B; 9.5–10 min, 5%–100% B; 10–11 min, 100% B; flow rate: 0.3 ml·min⁻¹. The HPLC eluate was administered to the MS system with a spray voltage of 1.0 kV. The MS peaks were recorded and compared with that of the Insp6 standard (phytic acid sodium salt hydrate, C₆H₁₈O₂₄P₆ · xNa⁺ · yH₂O, MERCK, P8810).