All animals were handled in strict accordance with good animal practice as defined by the relevant national and/or local animal welfare bodies, and all animal work was approved by the University of Minnesota Institutional Animal Care and Use Committee. Male heterozygous mice bearing an EGFP knock-in into the Brachyury locus (GFP/Bry+/−)46 (link) were mated with CD-1 IGS female mice (Charles River Laboratories). Eng-deficient embryos were generated by timed mating Eng+/− heterozygotes, or Engfl/Δ to Engwt/Δ, as previously described9 (link)47 (link). Dox-iEng embryos were generated by timed mating of iEng mice. All YSs were dissociated in 0.25% trypsin for 3 min at 37 °C.
Female cd 1 igs mice
Manufactured by Charles River Laboratories
Sourced in United States
Female CD-1-IGS mice are a commonly used outbred mouse strain. They are a general-purpose model suitable for a variety of research applications.
Automatically generated - may contain errors
7 protocols using female cd 1 igs mice
1
Genetic Mouse Embryo Dissociation
2
Intravenous Delivery of LPNP-p198 in Mice
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Five-week-old Crl: CD-1® IGS female mice, Charles River, Wilmington, MA, USA, were housed under specific pathogen-free conditions at a AAALAC (American Association for Accreditation of Laboratory Animal Care)-certified animal facility. All procedures related to the maintenance of animals and experiments were in strict accordance with the policy of the Internal Committee for the Care and Use of Animals (IACUC). Mice were randomly distributed into three experimental groups (four mice per group): saline solution (untreated), LPNP-p198, and LPNP-pCtrl. Administration was performed intravenously via the tail vein at a final dosing volume of 200 μL based on maximum bolus delivery volume for mice and at a dosing regimen of three times per week for six weeks. A daily general examination was carried out to determine any adverse effects caused by the treatment and to determine, if necessary, a humanitarian endpoint. After completion of the treatment regimen, blood parameters were assessed using a veterinary blood chemistry analyzer VetTest® 8008, IDEXX, Westbrook, ME, USA.
3
Preclinical Breast Cancer Models
4
CD-1 IGS Mice Protocols
5
Transgenic Mice for SARS-CoV-2 Research
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Female K18-hACE2 transgenic mice were purchased from Jackson laboratory and maintained in pathogen-free conditions and handling conforms to the requirements of the National Institutes of Health and the Scripps Research Institute Animal Research Committee. Harbor H2L2 human antibody transgenic mice (Harbor BioMed, Cambridge, MA) were utilized under a collaboration between the Icahn School of Medicine at Mount Sinai and Harbor BioMed. Eight-to twelve-week old mice were used. Animal studies were approved by the Icahn School of Medicine Institutional Animal Care and Use Committee (IACUC). Female CD-1-IGS mice (strain code #022) were obtained from Charles River at 6–8 weeks of age with a weight range of 22–28.8 g. Animal experiments were approved by Sorrento Therapeutics, Inc. Protocol approval committee and IACUC.
6
Transgenic Mouse Models for COVID-19 Research
7
Immunization and Colonization in Mice
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Female BALB/c (Charles River Laboratories) were used for all immunization experiments and female CD-1 IGS mice (Charles River Laboratories) were used for colonization studies in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, the national ‘Bundesgesetzblatt für die Republik Österreich'. The corresponding animal protocol (39/53/00 ex 2012/13) has been approved by the Austrian Federal Ministry of Science and Research Ref. II/10b and the local Committee on the Ethics of Animal Experiments of the University of Graz. Mice were housed with food and water ad libitum and monitored under the care of full-time staff and in accordance with the rules of the Institute of Molecular Biosciences at the University of Graz. All animals were acclimated for 1 week before any procedures were carried out and were 9–11 weeks old at the start of the experiment. Normal human serum was obtained and pooled from five healthy adult volunteers according to an approval of the University of Graz Ethics Commission (GZ. 39/31/63 ex 2012/13). All volunteers provided written consent.
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