The BCA assay was performed using the
Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. BALF was diluted 1:20–1:50 for BCA assays.
Albumin was quantified using a Mouse Albumin ELISA Kit (Bethyl/Fortis Life Sciences, Waltham, MA) according to the manufacturer’s instructions. BALF was diluted 1:10,000 for albumin ELISAs.
E-selectin was measured using the
Mouse E-selectin/CD62E DuoSet ELISA (R&D Systems) according to the manufacturer’s instructions. BALF was diluted 1:2.5–1:4 for E-selectin ELISAs.
Warheit-Niemi H.I., Huizinga G.P., Edwards S.J., Wang Y., Murray S.K., O’Dwyer D.N, & Moore B.B. (2022). Fibrotic Lung Disease Alters Neutrophil Trafficking and Promotes Neutrophil Elastase and Extracellular Trap Release. ImmunoHorizons, 6(12), 817-834.