Arrayscan vtireader

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ArrayScan VTIreader is a high-content imaging system designed for cell-based assays. It captures and analyzes images of cells to provide quantitative data on cellular responses and characteristics. The core function of the ArrayScan VTIreader is to perform automated image acquisition and analysis of cellular samples.

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Lab products found in correlation

Clear bottom 96 well plate, by Corning (2 mentions) Viewlux plate reader, by PerkinElmer (2 mentions) Celltiter glo reagent, by Promega (1 mentions)

4 protocols using arrayscan vtireader

Angiogenesis Co-Culture Assay Protocol

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The angiogenesis co-culture assay was conducted using the Angio-Ready
Angiogenesis Assay according to the manufacturer’s instructions. Cells were
seeded in a 96-well clear-bottom plate (Corning, Oneonta, NY) for 5 h and
exposed to test compounds for 3 days at 37 °C, 5% CO₂. The tube
formation and cell viability of each well were acquired on an ArrayScan VTI
reader (Thermo Fisher, Waltham, MA) with a 5× objective and
488_excitation/530_emission filters to image the green
fluorescent protein (GFP)–expressing tubular structures and on a ViewLux plate
reader (PerkinElmer, Waltham, MA) using CellTiter-Glo reagent (Promega, Madison,
WI), respectively.

Li S., Hsu C.W., Sakamuru S., Zou C., Huang R, & Xia M. (2017). Identification of Angiogenesis Inhibitors Using a Co-culture Cell Model in a High-Content and High-Throughput Screening Platform. Slas Technology, 23(3), 217-225.

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Angiogenesis Co-Culture Assay

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The angiogenesis co-culture assay was conducted using Angio-Ready Angiogenesis Assay (Cat. No. ACS-2001, ATCC) according to manufacturer instructions. Cells were seeded in a 96-well clear bottom plate (Corning) for five hours and exposed to test compounds for three days at 37°C, 5% CO₂. Tube formation and cell viability of each well were acquired on an ArrayScan VTI reader (Thermo Fisher Scientific) with a 5X objective and 488ex/530em filters to image the GFP-expressing tubular structures and on a ViewLux plate reader (PerkinElmer) using CellTiter-Glo reagent, respectively.

Hsu C.W., Huang R., Khuc T., Shou D., Bullock J., Grooby S., Griffin S., Zou C., Little A., Astley H, & Xia M. (2016). Identification of approved and investigational drugs that inhibit hypoxia-inducible factor-1 signaling. Oncotarget, 7(7), 8172-8183.

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3D Cell Line Spheroid Assay

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Spheroids were formed using HCT116GFP cell line, as described previously15 (link). Briefly, 10000 cells per well were seeded in 50 ml of fresh medium into 384-well F-bottom Corning Ultra-Low Attachment plates. Spheroids were cultured for 7 days without medium change prior to drug treatment. After treatment, cell viability was assessed by mean spheroid GFP fluorescence measurements, using ArrayScan VTI Reader (Cellomics Inc, Pittsburgh, PA, USA). The assay has been shown suitable for such measurements previously15 (link)16 (link).

Kashif M., Andersson C., Hassan S., Karlsson H., Senkowski W., Fryknäs M., Nygren P., Larsson R, & Gustafsson M.G. (2015). In vitro discovery of promising anti-cancer drug combinations using iterative maximisation of a therapeutic index. Scientific Reports, 5, 14118.

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High-throughput Screening of α2A-AR Agonists

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CHO-PKA-cat α_2A-AR cells were cultured in 96-cell plates and pre-treated with forskolin (10 μM) for 15 min, then incubated with candidate compounds. Cells were fixed and the formation of cytoplasmic spots were quantitatively measured using the Cellomics Array Scan VTI Reader and the Spot DetectorV3 BioApplication of a high-throughput screening assay. Activity was calculated as follows:
For positive controls, cells were pre-treated with forskolin for 15 min, then treated with the agonist DMED. Negative control cells were treated with 0.25% DMSO and forskolin. Furthermore, the antagonistic activity of compounds was tested in CHO-α_2A-PKAcatEGFP cells pre-treated with forskolin (10 μM) and DMED (10 μM).

Sun S., Li P., Wang J., Zhao D., Yang T., Zhou P., Su R., Zheng Z, & Li S. (2024). Novel Scaffold Agonists of the α2A Adrenergic Receptor Identified via Ensemble-Based Strategy. Molecules, 29(5), 1097.

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