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2 protocols using folin ciocalteu s fc reagent

1

Antioxidant Compound Extraction Protocol

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Gallic acid, Butylated hydroxy anisole (BHA), Quercetin, ferrous sulfate, Apocynin (4-Hydroxy-3-methoxy acetophenone), Vanillic acid, Dichloromethane (DCM), Polyvinyl alcohol (PVA) and Poly (lactic-co-glycolic acid) (PLGA) were procured from Sigma Aldrich, Bangalore, India. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Folin–Ciocalteu's (FC) reagent and were perchased from Hi-media Bengaluru, India. The other required chemicals and solvents used were of analytical grade.
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2

Antioxidant Activity Determination

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Sulphuric acid, sodium hydroxide, aluminium chloride, Folin-Ciocalteu’s (FC) reagent, gallic acid, DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tri(2-pyridyl)-s-triazine), ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid), and other chemicals were procured from Himedia Laboratories (Mumbai, India).
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