Fresh glioblastoma tumor tissues were cut into fragments and were repeatedly blown by suckers. After 1000 r/min centrifugation for 5 min, the red cell lysate was added to gently blow for 3 min. After another 1000 r/min centrifugation for 5 min, the supernatant was removed. Next, the cells were cultured using Cyagen Neural Stem Cell Basal Medium, including B27, Penicillin-Streptomycin, Glutamine, Heparin, bFGF, and EGF (Cyagen Biosciences, Suzhou, P. R. China). A large number of suspension cell balls appeared in primary culture after 7–10 days, after which, the cell spheres were dispersed into a single cell suspension and cultured each for 7 days. Primary GSCs (pGSCs) were identified by CD133 and Nestin staining. All procedures performed in this study were in accordance with the ethical standards of the institutional research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. This study was approved by the Ethics Committee of Nanjing Brain Hospital Affiliated to Nanjing Medical University and Affiliated Kunshan Hospital of Jiangsu University, and written informed consent was obtained from all patients providing tissue specimens.
Basic fibroblast growth factor (bfgf)
Manufactured by Cyagen
Sourced in United States
BFGF is a basic fibroblast growth factor, a type of protein that stimulates cell growth and division. It is commonly used in cell culture applications to support the proliferation of various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Epidermal growth factor (egf), by Cyagen (2 mentions)
Penicillin streptomycin, by Cyagen (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Accuri c5, by BD (1 mentions)
2 protocols using basic fibroblast growth factor (bfgf)
1
Isolation and Culture of Primary Glioblastoma Stem Cells
2
Cultivation and Characterization of Breast Cancer Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MCF-7 and MDA-MB-231 cells were obtained from ATCC (Manassas, VA, USA). They were cultivated in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS and 1% P/S (Gibco, USA) in an incubator with 5% CO2 at 37°C. Then, according to the culture method reported by Dontu et al. [19 (link), 20 (link)], the two kinds of breast cancer cells were plated in ultralow attachment 6-well plates with SFM including DMEM-F12 (Gibco), P/S, bFGF, EGF, B27 (Cyagen, USA), insulin, and BSA (Sigma-Aldrich). The growth medium was replenished every 2 days with fresh medium. Cells cultured under the condition forming nonattached tumor spheres were digested once a week. Third-generation spheres were collected to detect the CD44+CD24-/low cells by FCM (BD Accuri™ C5, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!