The largest database of trusted experimental protocols

Antibiotic and antimycotic solution

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Antibiotic and antimycotic solution is a sterile liquid composed of a combination of antibiotics and antifungal agents. It is designed for use in cell culture applications to prevent microbial contamination.

Automatically generated - may contain errors

2 protocols using antibiotic and antimycotic solution

1

EBV Virus Production for Rabbit Inoculation

Check if the same lab product or an alternative is used in the 5 most similar protocols
EBV producing B95-8 cells were cultured at 37 °C in RPMI (GIBCO) supplemented with 10% Fetal Bovine Serum (FBS) (GIBCO), 50 µg/ml gentamycin (Hyclone), 1% antibiotic and antimycotic solution (Santa Cruz) and 1X glutamine (GIBCO), as previously described24 (link). Cells were grown to a density of approximately 6.5 × 106cells/ml and then incubated at 30ºC for 24 h to promote viral production. The culture supernatant was centrifuged to remove cell debris and filtered using a 0.2 µm filter. Freshly filtered culture supernatant containing the virus was then used for intravenous inoculation of rabbits. Quantitative real-time PCR (qRT-PCR) was used to determine EBV copy number in the inoculum, using Namalwa cell line DNA as standards55 (link).
+ Open protocol
+ Expand
2

Culturing EBV Producer B95-8 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
EBV producer cell line (B95-8 cells) was cultured in RPMI media (GIBCO, Waltham, MA, USA), supplemented with 10% FBS (GIBCO, Waltham, MA, USA), 1% antibiotic and antimycotic solution (Santa Cruz, CA, USA), 50 µg/mL gentamycin (Hyclone, Logan, GA, USA), and 1× glutamine (GIBCO, Waltham, MA, USA) at 37 °C, and 5% CO2, as previously described [27 (link)]. Cells were cultured until they attained high density (6.5 × 106 cells/mL). Culture supernatants were centrifuged to remove cells/cell debris and then filtered using a 0.2 μm filter. Fresh-filtered virus preparations were used for inoculating rabbits. EBV copy number in the inoculum was estimated by quantitative real-time PCR (qPCR) [27 (link)].
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!