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Chlorophyll meter model spad 502

Manufactured by Konica Minolta
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Sourced in Japan

The Chlorophyll Meter Model (SPAD-502) is a compact, handheld device designed to measure the relative chlorophyll content in plant leaves. It utilizes dual-wavelength spectroscopy to provide a numerical value that represents the chlorophyll concentration in the leaf sample.

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Lab products found in correlation

Li 6400xt photosynthesis system, by LI COR (1 mentions) Li 6400, by LI COR (1 mentions) Li 3100c, by LI COR (1 mentions)

4 protocols using chlorophyll meter model spad 502

1

Gas Exchange Measurements and Photosynthesis

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Gas exchange parameters, including the stomatal conductance (gs) and intercellular carbon dioxide concentration (Ci), were measured by an LI-6400XT photosynthesis system (LI-COR Inc., Lincoln, NE, USA) from 9:00-11:00. The leaf chamber temperature was set to 25°C, the relative humidity was set to 65%, and the CO2 concentration was kept at 390 μmol mol–1. Photosynthetically active radiation (PAR) was set to 1800, 1600, 1400, 1200, 1000, 800, 600, 400, 200, 150, 100, 50, and 0 μmol m-2 s-1, respectively. The stomatal limit (Ls) value was determined by equation (1).
Where Ca indicates the atmospheric CO2 concentration.
And
The PNmax was obtained by the photosynthesis-light response curves based on the photosynthetic electron transport of photosystem II in C3 and C4 species (Ye, 2012 (link); Ye et al., 2013 (link)).
SPAD value was measured by Chlorophyll Meter Model (SPAD-502, Konica Minolta, Japan).
Luo J., Yang Z., Zhang F, & Li C. (2023). Effect of nitrogen application on enhancing high-temperature stress tolerance of tomato plants during the flowering and fruiting stage. Frontiers in Plant Science, 14, 1172078.
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2

Leaf Photosynthesis and Chlorophyll Content

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The net photosynthetic rate of leaves was measured at the same time using a Li-6400 portable photosynthesizer (LI-COR 6400, Lincoln, USA) with the following parameters set: light quantum flux at 1200 umol/(m2·s), CO2 concentration at 450 umol/m2, flow rate at 500 umol/s, and temperature at 25°C. Ten plants were measured during each treatment. The leaf chlorophyll content, denoted by the SPAD value, was measured on a SPAD meter (Chlorophyll Meter Model SPAD-502, Konica Minolta Inc, Japan) every 7 days at 9-11 am after different night temperature treatments (Ergo et al., 2018 (link)).
Yang L., Song W., Xu C., Sapey E., Jiang D, & Wu C. (2023). Effects of high night temperature on soybean yield and compositions. Frontiers in Plant Science, 14, 1065604.
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3

Photosynthesis and Chlorophyll Measurement

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Photosynthetic rate was measured according to Gai et al.23 (link). Photosynthetic rate was determined by using the CI-340 portable photosynthesis measuring system (CID, Inc., USA).
Measurement of chlorophyll content (SPAD) was conducted by using SPAD-502 Chlorophyll Meter Model (Konica Minolta Inc., Japan).
Gai Z., Liu L., Zhang J., Liu J, & Cai L. (2020). Effects of exogenous α-oxoglutarate on proline accumulation, ammonium assimilation and photosynthesis of soybean seedling (Glycine max(L.) Merr.) exposed to cold stress. Scientific Reports, 10, 17017.
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4

Comparative analysis of diploid and tetraploid plant growth

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Plant height (H) of 1 month old 2x and 4x plants was measured from the apex to the base using a ruler once a month. Total leaf greenness was measured in the 1st, 5th, 10th, 15th, and 20th leaves of 8 month old plants using a SPAD-502 Chlorophyll Meter Model (Minolta, Tokyo, Japan). Leaf area and Pn (net photosynthetic rate) were measured from the first fully expanded leaf on the stem (the diploids were marked as D_1, D_2, D_3, D_20, while the tetraploids were marked as T_1, T_2, T_3, T_20 from the top to the bottom of the stem). Leaf areas were measured using a portable area meter (LI-3100C, LI-COR, Lincoln, NE, USA). Pn was measured using the LI-6400-02B portable photosynthesis system (Li-COR-6400, Li-COR, Inc., Lincoln, NE, USA). Measurements were taken on sunny days at 8:30–11:30 a.m., with maintenance of the photosynthetic photon flux density at 1400 mol (photon)·m−2·s−1, a CO2 concentration of 400 mol·mol−1, relative air humidity of 60–65%, and flow of 500 mol·mol−1. Three biological replicates were used for each genotype.
Xu C., Zhang Y., Han Q, & Kang X. (2020). Molecular Mechanism of Slow Vegetative Growth in Populus Tetraploid. Genes, 11(12), 1417.
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