Annexin 5 fitc

Flow cytometric and TUNEL assays were respectively used to determine the potential influence of DHA on T cell apoptosis. For flow cytometry, CD4+ T cells purified with MACS beads (Miltenyi Biotec) were stimulated with plate-bound anti-CD3e and anti-CD28 for 72 h, and then stained with Annexin V-FITC and PI according to the manufacturer’s protocols (Absin, Shanghai, China) and analyzed by flow cytometry.
For TUNEL staining, slides were stained with the reagents supplied by the TUNEL Kit (Roche, Switzerland). All TUNEL-positive cells assessed in three different unit areas were counted. The average number of cells per unit area number for each set of specimens in each group was calculated and compared.

Cells were seeded in wells of 6-well plates with 15×10⁴cells/well and incubated in DMEM containing 10% FBS. The OAS1-siRNAs were used to impair OAS1 expression for 48 h, then cells were treated with 10 μM cisplatin for 24h. Finally, cells were digested and re-suspension with 1×bingding buffer, then, cells were treated with Annexin V-FITC (10 μl) and PI (5μl) (Absin, China) (26 (link)).