Rabbit anti trpm7

Manufactured by Abcam

Sourced in China

Rabbit anti-TRPM7 is a primary antibody that specifically recognizes the TRPM7 protein. TRPM7 is a member of the transient receptor potential (TRP) ion channel family and functions as a calcium-permeable cation channel.

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Anti-TRPM7 (18 citations) TRPM7 (11 citations) Rabbit anti- (5 citations)

2 protocols using rabbit anti trpm7

Immunohistochemical Profiling of Cellular Markers

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The dewaxed slide was treated with Proteinase K (Sigma-Aldrich) for proteolytic digestion and 3% H₂O₂ for the elimination of endogenous peroxidase activity. After blocking with normal goat serum, the slides were incubated with primary antibody overnight at 4 °C, respectively. The primary antibodies used in this study include: rabbit anti-OCN (Abcam), rabbit anti-IL-8 (Abcam), rabbit anti-CCL5 (Abcam), rabbit anti-IL-1β (Abcam), rabbit anti-IL-1ra (Abcam), anti-CD68 (Abcam), rabbit anti-Phospho-Histone H3S10 (Abcam), and rabbit anti-TRPM7 (Abcam). The slides were then incubated with goat anti-rabbit secondary antibody and visualized using Diaminobenzidine (DAB) staining kit (Santa Cruz Biotechnology, Santa Cruz, USA) following the manufacturer’s instruction. Immunofluorescent staining was done using Alexa-Fluor 488 conjugated anti-rabbit IgG or Alexa-Fluor 647 conjugated anti-mouse IgG secondary antibodies (Thermo Fisher Scientific) and Hoechst 33324 (Thermo Fisher Scientific). Immunofluorescent images were captured using an LSM 780 confocal microscopy (Zeiss, Germany)

Qiao W., Wong K.H., Shen J., Wang W., Wu J., Li J., Lin Z., Chen Z., Matinlinna J.P., Zheng Y., Wu S., Liu X., Lai K.P., Chen Z., Lam Y.W., Cheung K.M, & Yeung K.W. (2021). TRPM7 kinase-mediated immunomodulation in macrophage plays a central role in magnesium ion-induced bone regeneration. Nature Communications, 12, 2885.

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Protein Extraction and Western Blotting

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Total protein was extracted from RPE cells using radioimmunoprecipitation assay (RIPA) buffer supplemented with phenylmethylsulfonyl fluoride (PMSF) at the ratio of 100:1, and quantified using the bicinchoninic acid (BCA) assay. Equal amounts of protein per sample were separated through 10% to 15% sodium dodecyl sulfate polyacrylamide (SDS-PA) gel, and the bands were transferred onto polyvinylidene difluoride (PVDF) membranes according to the manufacturer's protocol. After blocking with a commercially available blocking buffer (Beyotime, Shanghai, China), the blots were incubated overnight with primary antibodies (rabbit anti-TRPM7, rabbit anti-PKC, rabbit anti-ERK, and rabbit anti-GAPDH primary antibodies, Abcam, UK) at 4°C.

Hu L, & Xu G. (2021). Potential Protective Role of TRPM7 and Involvement of PKC/ERK Pathway in Blue Light–Induced Apoptosis in Retinal Pigment Epithelium Cells in Vitro. Asia-Pacific Journal of Ophthalmology (Philadelphia, Pa.), 10(6), 572-578.

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