Silica gel 60 f254 aluminum backed plates

Manufactured by Merck Group

Sourced in Germany

Silica Gel 60 F254 aluminum-backed plates are a type of thin-layer chromatography (TLC) media. They consist of a thin layer of silica gel coated on an aluminum backing. The silica gel layer contains a fluorescent indicator (F254) that allows for the visualization of separated compounds under ultraviolet (UV) light.

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Lab products found in correlation

Amx 3 400 spectrometer, by Bruker (1 mentions) Silica gel 60 0.040 0 063 mm, by Merck Group (1 mentions) Silica gel 60 f254 glass backed plates, by Merck Group (1 mentions) Avance 3 hd 400, by Bruker (1 mentions)

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Silica gel 60 (932 citations) Silica gel 60 plates (129 citations) Silica gel plates (98 citations) F254 plates (71 citations) 60 F254 plates (65 citations) Silica gel F254 (52 citations) Silica plates (15 citations) Silica gel 60 aluminum plates (5 citations) Aluminum-backed silica plates (2 citations) 60 F254 silica (2 citations)

2 protocols using silica gel 60 f254 aluminum backed plates

Organic Synthesis Techniques and Instrumentation

Cited 1 time

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Commercial reagents Acros (Geel, Belgium), Sigma Aldrich (St. Louis, MO, USA) and Fluka (Bucharest, Romania) were used for the reactions; anhydrous solvents of high quality were used without additional purification. Silica Gel 60 0.040–0.063 mm (Merck, Darmstadt, Germany) was used for column chromatography. Silica Gel 60 F₂₅₄ aluminum-backed plates (Merck, Darmstadt, Germany) were used for thin layer chromatography (TLC), and Silica Gel 60 F₂₅₄ glass-backed plates (Merck, Darmstadt, Germany) for preparative layer chromatography (PLC). NMR spectra were registered on an AMX III-400 spectrometer (Bruker, Newark, Germany) with the working frequency of 400 MHz for ¹H NMR (Me₄Si as an internal standard for organic solvents) and 100.6 MHz for ¹³C NMR (with carbon-proton interaction decoupling).
High resolution mass spectra were measured on Bruker micrOTOF II instruments using electrospray ionization (ESI HRMS) using standart published conditions [24 (link)].

Khandazhinskaya A.L., Mercurio V., Maslova A.A., Palomino R.A., Novikov M.S., Matyugina E.S., Paramonova M.P., Kukhanova M.K., Fedorova N.E., Yurlov K.I., Kushch A.A., Tarasova O., Margolis L., Kochetkov S.N, & Vanpouille C. (2021). Dual-targeted anti-CMV/anti-HIV-1 heterodimers. Biochimie, 189, 169-180.

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Synthesis and Characterization of Azobenzene Compounds

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All reagents and solvents were purchased
from commercial sources (Sigma-Aldrich, TCI Europe N.V., Strem Chemicals,
etc.) and were used without further purification. Solvents were obtained
from Fisher Scientific. Reactions were monitored by thin layer chromatography
(TLC) on precoated, Merck Silica gel 60 F₂₅₄ aluminum-backed
plates, and the chromatograms were first visualized by UV irradiation
at λ = 254 nm. Flash silica gel chromatography was performed
using silica gel (SiO₂, particle size 40–63 μm)
purchased from SiliCycle. NMR spectra were measured on a BRUKER Avance
III HD 400 (equipped with a CryoProbe). Multiplicities in the following
experimental procedures are abbreviated as follows: s = singlet, d
= doublet, t = triplet, q = quartet, m = multiplet. ¹H
chemical shifts are expressed in parts per million (ppm, δ scale)
and are referenced to the residual protium in the NMR solvent (CDCl₃: δ = 7.26; THF-d₈: δ
= 1.72). ¹³C chemical shifts are expressed in ppm (δ
scale) and are referenced to the carbon resonance of the NMR solvent
(CDCl₃: δ = 77.16, THF-d₈: δ = 25.31). NOTE: Due to the trans/cis isomerization of some compounds containing an azobenzene
functionality, more signals were observed in the ¹H and ¹³C spectra than would be expected for the pure trans-isomer. Only signals for the major trans-isomer
are reported.

Tei R., Morstein J., Shemet A., Trauner D, & Baskin J.M. (2021). Optical Control of Phosphatidic Acid Signaling. ACS Central Science, 7(7), 1205-1215.

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