Fc receptor binding was blocked by incubation with anti-CD16/CD32 antibodies (clone 93, eBioscience) for 5 min at RT. The cells were then incubated for 30 min at 4 °C with specific antibodies (anti-CD3e/APC (145-2C11), anti-CD4/FITC (GK1.5), anti-CD8/eFluor506 (53–6.7), anti-CD25/PE-Cy7 (PC61.5), (eBioscience); anti-CD3/PE (17A2); anti-CD69/Pacific blue (H1.2F3); anti-B220/ PE-Cy7 (RA3-6B2) (Biolegend (San Diego, CA); anti-IgA/FITC (C10–3) (BD Biosciences) and CD138/APC (REA104) (Miltenyi Biotec) in PBS/ 3% FBS, washed and resuspended in cold PBS. The data were acquired by a CytoFLEX (Beckman Coulter) flow cytometer and analyzed using FlowJo software.
Anti iga fitc c10 3
Manufactured by BD
Anti-IgA-FITC (C10-3) is a fluorescently labeled antibody that specifically binds to human IgA. It is used for the detection and quantification of IgA in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti penta his 34660, by Qiagen (2 mentions)
Anti tubulin dm1a, by Merck Group (2 mentions)
Anti igg1 apc x56, by BD (2 mentions)
Anti gfp hrp b 2, by Santa Cruz Biotechnology (2 mentions)
Anti flag m2, by Merck Group (2 mentions)
Anti cd16 cd32 antibodies clone 93, by Thermo Fisher Scientific (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
I5006, by Merck Group (1 mentions)
Ab1791, by Abcam (1 mentions)
3 protocols using anti iga fitc c10 3
1
Multicolor Flow Cytometry for Immune Cell Analysis
2
Polyclonal SANBR Antibody Production
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The polyclonal SANBR antibody was produced by immunizing rabbits with the C-terminal SANBR peptide (RSKSRFGQGRPA) and isolating reactive antibodies from sera (Covance). Antibodies for flow cytometry include: anti-IgG1-APC (X56, BD Pharmingen), anti-IgA-FITC (C10-3, BD Pharmingen). Antibodies for immunoblot include: anti-AID (53 (link)), anti-tubulin (DM1A, Sigma), anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2, Santa Cruz), anti-Flag (M2, Sigma). Antibodies for chromatin immunoprecipitation (ChIP) include: anti-H3 (ab1791, Abcam) and rabbit IgG (I5006, Sigma).
3
KIAA1841 Antibody Production and Validation
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The polyclonal KIAA1841 antibody was produced by immunizing rabbits with the C-terminal KIAA1841 peptide (RSKSRFGQGRPA) and isolating reactive antibodies from sera (Covance).
Antibodies for flow cytometry include: anti-IgG1-APC (X56, BD Pharmingen), anti-IgA-FITC (C10-3, BD Pharmingen). Antibodies for immunoblot include: anti-AID 53 , anti-tubulin (DM1A, Sigma), anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2, Santa Cruz), anti-Flag (M2, Sigma).
Antibodies for flow cytometry include: anti-IgG1-APC (X56, BD Pharmingen), anti-IgA-FITC (C10-3, BD Pharmingen). Antibodies for immunoblot include: anti-AID 53 , anti-tubulin (DM1A, Sigma), anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2, Santa Cruz), anti-Flag (M2, Sigma).
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