Single-cell suspensions from spleen and inguinal lymph nodes were prepared in complete culture medium (Dulbecco’s modified eagle’s medium + GlutaMAX-I, 1 % fetal bovine serum, 1 mM Hepes, 5 μM 2- mercaptoethanol, and penicillin/streptomycin (50,000 units/10,000 μg)). All reagents were purchased from Invitrogen, Thermo Fisher Scientific, Waltham, MA. Red blood cells were lysed (BD Pharm Lysing Buffer, BD Biosciences, San Jose, CA) in spleen samples. Splenic and inguinal lymph node lymphocytes from naïve and MOG35-55 immunized mice were stained with anti-mouse CD4-phycoerythrin (PE) (BD Biosciences, San Jose, CA), anti-mouse CD8-PE-cyanine5 (CY5) (BD Biosciences, San Jose, CA), and anti-mouse CD19- fluorescein isothiocyanate (FITC) (BD Pharmingen, San Diego, CA), and fixed with Cytofix Fixation Buffer (BD Biosciences, San Jose, CA) for measurements on a FACS Calibur (BD Biosciences, San Jose, CA) or Gallios Flow Cytometer (Beckman Coulter, Brea, CA). Data was analyzed with the FlowJo soware.
Anti mouse cd8 pe cyanine5 cy5
Manufactured by BD
The Anti-mouse CD8-PE-cyanine5 (CY5) is a fluorescently labeled antibody that binds to the CD8 receptor on the surface of mouse T cells. It can be used for the identification and quantification of CD8+ T cells in flow cytometry applications.
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Lab products found in correlation
2 protocols using anti mouse cd8 pe cyanine5 cy5
1
Immunophenotyping of Lymphocytes in EAE
2
Immunophenotyping of Lymphocytes in EAE
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Single-cell suspensions from spleen and inguinal lymph nodes were prepared in complete culture medium (Dulbecco’s modified eagle’s medium + GlutaMAX-I, 1 % fetal bovine serum, 1 mM Hepes, 5 μM 2- mercaptoethanol, and penicillin/streptomycin (50,000 units/10,000 μg)). All reagents were purchased from Invitrogen, Thermo Fisher Scientific, Waltham, MA. Red blood cells were lysed (BD Pharm Lysing Buffer, BD Biosciences, San Jose, CA) in spleen samples. Splenic and inguinal lymph node lymphocytes from naïve and MOG35-55 immunized mice were stained with anti-mouse CD4-phycoerythrin (PE) (BD Biosciences, San Jose, CA), anti-mouse CD8-PE-cyanine5 (CY5) (BD Biosciences, San Jose, CA), and anti-mouse CD19- fluorescein isothiocyanate (FITC) (BD Pharmingen, San Diego, CA), and fixed with Cytofix Fixation Buffer (BD Biosciences, San Jose, CA) for measurements on a FACS Calibur (BD Biosciences, San Jose, CA) or Gallios Flow Cytometer (Beckman Coulter, Brea, CA). Data was analyzed with the FlowJo soware.
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