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Z0334

Manufactured by Novus Biologicals
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Z0334 is a laboratory equipment product from Novus Biologicals. It is designed for use in research and scientific applications. The core function of this product is to provide a specific capability or tool for conducting experiments or analyses, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

D9564, by Merck Group (2 mentions) Prb 278p, by Merck Group (2 mentions) Prb 435p, by Merck Group (2 mentions) Adi spa 223, by GeneTex (2 mentions) Ab15766, by GeneTex (2 mentions) Gtx62440, by Merck Group (2 mentions) Gtx11268, by Fortrea (2 mentions) Mab377, by Fortrea (2 mentions)

2 protocols using z0334

1

Immunofluorescence Staining of Neural Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde (PFA) for 10 min, followed by washes in PBS at room temperature (RT). Cells were then blocked with 3% donkey serum in PBS containing 0.01% Triton X-100 for 1 hr at RT, incubated with primary antibodies overnight at 4°C, and then washed with PBS and incubated with secondary antibodies for 1 hr at RT. We used primary antibodies for GFAP (1:2,000; DAKO; Z0334), S100β (1:200; NOVUS; NB110–57478), ALDH1L1 (1:200; NeuroMab; 75–140), synapsin (1:1,000; SYSY; 106103), αB-crystallin (1:200; Enzo; ADI-SPA-223), MAP2 (1:500; GeneTex; GTX11268), Tuj1 (1:6,000; Covance; PRB-435P), NeuN (1:400; Millipore; MAB377), Pax6 (1:500; Covance; PRB-278P), Sox1 (1:500; Millipore; AB15766), Oligo2 (1:200; GeneTex; GTX62440), NG2 (1:500; Millipore; MAB5384), and NKX2.2 (1:50; DSHB; 745A5). Nuclei were stained with DAPI (1:6,000; Sigma; D9564).
Tian E., Sun G., Sun G., Chao J., Ye P., Warden C., Riggs A.D, & Shi Y. (2016). Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes. Cell reports, 16(3), 781-792.
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2

Immunofluorescence Staining of Neural Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde (PFA) for 10 min, followed by washes in PBS at room temperature (RT). Cells were then blocked with 3% donkey serum in PBS containing 0.01% Triton X-100 for 1 hr at RT, incubated with primary antibodies overnight at 4°C, and then washed with PBS and incubated with secondary antibodies for 1 hr at RT. We used primary antibodies for GFAP (1:2,000; DAKO; Z0334), S100β (1:200; NOVUS; NB110–57478), ALDH1L1 (1:200; NeuroMab; 75–140), synapsin (1:1,000; SYSY; 106103), αB-crystallin (1:200; Enzo; ADI-SPA-223), MAP2 (1:500; GeneTex; GTX11268), Tuj1 (1:6,000; Covance; PRB-435P), NeuN (1:400; Millipore; MAB377), Pax6 (1:500; Covance; PRB-278P), Sox1 (1:500; Millipore; AB15766), Oligo2 (1:200; GeneTex; GTX62440), NG2 (1:500; Millipore; MAB5384), and NKX2.2 (1:50; DSHB; 745A5). Nuclei were stained with DAPI (1:6,000; Sigma; D9564).
Tian E., Sun G., Sun G., Chao J., Ye P., Warden C., Riggs A.D, & Shi Y. (2016). Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes. Cell reports, 16(3), 781-792.
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