Anti c myc

Cells were collected, washed, and smeared on the slides. The cells were fixed with 4% paraformaldehyde, permeabilized with 1% Triton X-100, and blocked with goat serum. After that, the cells were incubated with corresponding primary antibodies, including anti-c-abl (Santa USA), anti-STAT6 (Abcam, USA), anti-p-STAT6 (CST, USA), and anti-c-Myc (Abmart, China), at 4 °C overnight. The next day, the cells were incubated with Cy-3 or FITC labeled secondary antibody (Invitrogen, USA). Then, the nucleus was stained with 4.6-diamidino-2-phenylindole (DAPI). Finally, the slides were sealed with glycerin and observed with the confocal microscope.

Total protein samples were extracted from OC cell lines and tissues using RIPA (Cat No. BL504A, Biosharp, China) lysis buffer supplemented with protease/phosphatase inhibitors (Cat No. BL612A, Biosharp, China). Proteins (20 μg/lane) were separated using 10–12% SDS‒PAGE and then transferred onto a PVDF membrane (Cat No. IPVH00010, Millipore, USA). The membrane was then blocked in 5% nonfat milk and incubated overnight with specific primary antibodies at 4 °C. The primary antibodies included anti-β-Actin (1:50,000; Cat No. AC026, ABclonal, Wuhan, China), anti-GAPDH (1:2000; Cat No. 60004-1-Ig, Proteintech, Wuhan, China), anti-CD24 (1:1000; Cat No. AP22227c, Abcepta, Suzhou, China), anti-CD47 (1:1000; Cat No. EM1701-37, HUABIO, Hangzhou, China), anti-HIF-1α (1:2000; Cat No. 79233, CST, USA), anti-p-STAT3 (1:1000; Cat No. 9145, CST, USA), anti-STAT3 (1:1000; Cat No. 4904, CST, USA), anti-Caspase 3/p17/p19 (1:1000; Cat No. 19677-I-AP, Proteintech, Wuhan, China) and anti-C-Myc (1:1000; Cat No. T55150, Abmart, Shanghai, China). After incubation with the corresponding secondary antibodies, bands were detected using ECL chemiluminescence substrate (Cat No. BL520A, Biosharp, China).