Extracts B and C were analyzed via gas chromatography/mass spectrometry (GC-MS) analysis. After dissolving samples in methanol, GC analyses were achieved by using a Shimadzu GC17AGC equipped with HP 5MS column and a flame ionization detector (FID) controlled by Borwin Software (Shimadzu, Milan, Italy). GC/MS analyses were completed by using a Hewlett-Packard 6890 gas chromatograph connected to a Hewlett-Packard 5973 Mass Selective, using electron impact (EI) (Agilent Technologies, Milan, Italy). Injector and detector temperatures were maintained at 250 °C. The initial oven temperature was set at 50 °C for 3 min. The temperature rate was set on 10 °C min−1 up to 250 °C. The extracts (1 g) were subjected to flash column chromatography purification (n-hexane/ethyl acetate), in order to isolate the main components. 1H-NMR and 13C-NMR spectra of the recovered compounds were detailed on a Bruker 300 MHz spectrometer. Chemical shifts (δ) were reported in ppm, using tetramethylsilane (TMS) as the internal reference standard. Coupling constants were reported as a J value in Hertz (Hz).
Gc17agc
Manufactured by Shimadzu
Sourced in Italy
The GC17AGC is a gas chromatograph (GC) manufactured by Shimadzu. It is designed for the analysis and separation of chemical compounds in a sample. The GC17AGC utilizes a column and a detector to identify and quantify the components of a mixture.
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3 protocols using gc17agc
1
Analytical Characterization of Bioactive Compounds
2
Honey Composition Analysis by GC/GC-MS
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Gas chromatography (GC) analyses were performed after dissolving the honey samples in methanol by using a Shimadzu GC17AGC equipped with a flame ionization detector (FID) controlled by Borwin Software (Shimadzu, Milan, Italy). Gas chromatography/mass spectrometry (GC/MS) analyses were performed by using a Hewlett-Packard 6890 gas chromatograph interfaced with a Hewlett-Packard 5973 Mass Selective, using electron impact (EI) (Agilent Technologies, Milan, Italy). The temperature of both the injector and detector was 250 °C. The initial oven temperature was set at 50 °C for three minutes. The temperature rate was set on 10 °C min−1 up to 250 °C.
3
Analysis of Tcmucins and GIPL Structures
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All solvents were purchased from Tedia (Fairfield, OH, USA). Resins and columns were acquired from BioRad (Richmond, CA, USA), and Restek (Bellefonte, PA, USA). Other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gas chromatography coupled with mass spectrometry (GC-MS) experiments were run in a Shimadzu GC-17A GC coupled with a Shimadzu GCMS-QP5050A mass spectrometer.
NMR experiments for the analysis of Tcmucins and GIPL structures were carried out on a Bruker Ascend 500 MHz spectrometer equipped with a 5 mm BBI gradient probe at the Centro Nacional de Ressonância Magnética Nuclear, UFRJ, Brazil.
NMR experiments for the analysis of Tcmucins and GIPL structures were carried out on a Bruker Ascend 500 MHz spectrometer equipped with a 5 mm BBI gradient probe at the Centro Nacional de Ressonância Magnética Nuclear, UFRJ, Brazil.
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