Hybridization chain reaction (HCR) and Whole Mount Immunohistochemistry were done in accordance with previous described methods [42 (link), 61 (link)]. The HCR probes transcripts were synthesized by Molecular Instruments for ret, NM_181662.2; gfra1a, NM_131730.1; oprl1, NM_205589.2; oprd1b, NM_131258.4; etv1, XM_005157634.4; elavl3, NM_131449. The following primary antibodies were used: goat polyclonal IgG anti-Choline Acetyltransferase (ChaT, Millipore Sigma, AB144P, 1:500), rabbit polyclonal IgG anti-5-HT (serotonin, Immunostar, 20080, 1:250), mouse monoclonal IgG2b anti-HuC/D (Invitrogen Thermo Fisher, A-21271, 1:250), Mouse monoclonal IgG1 anti-Phox2b (B-11, Santa Cruz Biotechnology, SC-376997, 1:250). The following secondary antibodies were used from Invitrogen: Alexa Fluor 488 donkey anti-goat IgG (A-11055, 1:600), Alexa Fluor 405 goat anti-rabbit IgG (A-48254, 1:600), Alexa Fluor 647 goat anti-mouse IgG2b (A-21242, 1:600), Alexa Fluor 594 goat anti-mouse IgG1 (A-21125, 1:600). High content semi-automated confocal imaging and processing was done as described above.
Gfra1a
Manufactured by Molecular Devices
Gfra1a is a laboratory equipment product. It is a protein that plays a core role in the GDNF signaling pathway, which is involved in the regulation of neuronal development and survival.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 donkey anti goat igg, by Thermo Fisher Scientific (2 mentions)
Goat anti mouse igg2b alexa fluor 647, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 594 goat anti mouse igg1, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 405 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions)
Oprl1, by Molecular Devices (2 mentions)
Oprd1b, by Molecular Devices (2 mentions)
Elavl3, by Molecular Devices (2 mentions)
Mouse monoclonal igg2b anti huc d, by Thermo Fisher Scientific (2 mentions)
Mouse monoclonal igg1 anti phox2b b 11, by Santa Cruz Biotechnology (2 mentions)
2 protocols using gfra1a
1
Multimodal profiling of neuronal cell types
2
Whole-Mount Immunofluorescence and HCR for Zebrafish Neuroanatomy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Hybridization chain reaction (HCR) and Whole Mount Immunofluorescence were done in accordance with previous described methods (Ibarra-García-Padilla et al., 2021 (link); Uribe and Bronner, 2015 (link)). The HCR probes transcripts were synthesized by Molecular Instruments for ret, NM_181662.2; gfra1a, NM_131730.1; oprl1, NM_205589.2; oprd1b, NM_131258.4; etv1, XM_005157634.4; elavl3, NM_131449. The following primary antibodies were used: goat polyclonal IgG anti-Choline Acetyltransferase (ChaT, Millipore Sigma, AB144P, 1:500), rabbit polyclonal IgG anti-5-HT (serotonin, Immunostar, 20080, 1:250), mouse monoclonal IgG2b anti-HuC/D (Invitrogen Thermo Fisher, A-21271, 1:250), Mouse monoclonal IgG1 anti-Phox2b (B-11, Santa Cruz Biotechnology, SC-376997, 1:250). The following secondary antibodies were used from Invitrogen: Alexa Fluor 488 donkey anti-goat IgG (A-11055, 1:600), Alexa Fluor 405 goat anti-rabbit IgG (A-48254, 1:600), Alexa Fluor 647 goat anti-mouse IgG2b (A-21242, 1:600), Alexa Fluor 594 goat anti-mouse IgG1 (A-21125, 1:600). High content semi-automated confocal imaging and processing was done as described above.
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