Pe mouse anti human γδ tcr

Manufactured by BD

The PE Mouse Anti-Human γδ TCR is a laboratory reagent used for the detection and identification of human gamma-delta T cell receptor (γδ TCR) expressing cells. This monoclonal antibody is conjugated with the fluorescent dye Phycoerythrin (PE) for flow cytometric analysis.

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Lab products found in correlation

Bv421 mouse anti human cd3, by BD (2 mentions) Ebioscience fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Facsymphony, by BD (1 mentions) Annexin 5 apc, by BioLegend (1 mentions)

Spelling variants of this product

γδ TCR (11 citations) Anti-γδ TCR PE (3 citations) Anti-γδ TCR (2 citations) Mouse anti (2 citations)

2 protocols using pe mouse anti human γδ tcr

Flow Cytometry Analysis of Immune Cells

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Cells were washed with phosphate buffer saline (PBS) and spun at 300 × g in flow cytometry tubes. The supernatant was decanted and replaced with eBioscience Fixable Viability Dye eFluor780 (ThermoFisher) for 30 min. Cells were washed in 10 × PBS and resuspended with the appropriate antibodies. Antibodies from BD Biosciences include: BV421 Mouse Anti-Human CD3 (Clone UCHT1), PE Mouse Anti-Human γδ TCR (Clone 11F2), BUV395 Mouse Anti-Human CD56 (Clone NCAM16.2), BUV395 Mouse Anti-Human CD56, BV711 Mouse Anti-Human CD178 (Clone NOK-1), BV786 Mouse Anti-Human CD107a (Clone H4A3), BV480 Mouse Anti-Human CD3 (Clone UCHT1), APC-R700 Mouse Anti-Human CD56 (Clone NCAM16.2), BV711 Mouse Anti-Human CD27 (Clone M-T271), BUV496 Mouse Anti-Human CD16 (Clone 3G8), BV421 Mouse Anti-Human CD57 (Clone NK-1), BV786 Mouse Anti-Human PD1 (Clone EH12.1), PE-CF594 Mouse Anti-Human PDL1 (Clone MIH1), BUV737 Mouse Anti-Human FAS (Clone DX2), PE Mouse Anti-Human FASL (Clone NOK-1), and BUV395 Mouse Anti-Human CD107a (Clone H4A3). Cells were analyzed using a LSRII (BD Biosciences), an Aurora (CYTEK), and a BD FACSymphony (BD Biosciences).

Burnham R.E., Zoine J.T., Story J.Y., Garimalla S.N., Gibson G., Rae A., Williams E., Bixby L., Archer D., Doering C.B, & Spencer H.T. (2020). Characterization of Donor Variability for γδ T Cell ex vivo Expansion and Development of an Allogeneic γδ T Cell Immunotherapy. Frontiers in Medicine, 7, 588453.

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Flow Cytometry of Immune Cells

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To prepare cells for flow cytometry, cells were washed with 10 X phosphate buffer saline (PBS) and spun at 300 x g. Cells were stained with eBioscience Fixable Viabililty dye eFluor780 (ThermoFisher) for 30 minutes. After incubation with the viability dye, cells were washed in 10 X PBS and resuspended with the appropriate antibodies. Antibodies from BD Biosciences include: BV421 Mouse Anti-Human CD3 (Clone UCHT1, 5 µL/100 µL sample), PE Mouse Anti-Human γδ TCR (Clone 11F2, 5 µL/100 µL sample for a final concentration of 1.25 µg/mL), and APC-R700 Mouse Anti-Human CD56 (Clone NCAM16.2, 5 µL/100 µL sample). APC Annexin V was acquired from Biolegend. Cells were analyzed using an Aurora (CYTEK) flow cytometer.

Burnham R.E., Tope D., Branella G., Williams E., Doering C.B, & Spencer H.T. (2021). Human serum albumin and chromatin condensation rescue ex vivo expanded γδ T cells from the effects of cryopreservation. Cryobiology, 99, 78-87.

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