MicroPET and microCT imaging was performed as described previously [19 (link)]. A range of 89ZrmalDFO-GK1.5 cDb doses (2–40 µg; 0.26–5.2 MBq) were prepared in 100 µl saline and injected intravenously into mice. Mice were anesthetized with 2% isoflurane and PET and CT images were acquired 20 h post-injection. MicroPET images were acquired on an Inveon PET (Siemens) scanner for 600 sec and reconstructed using a 3D-ordered subset expectation maximization (OSEM) algorithm with 2 iterations, followed by maximum a posteriori (MAP) with 18 iterations (beta=0.1) with a zoom factor of 2.1, without attenuation correction. Whole-body CT images were acquired using the MicroCAT II (Imtek) scanner, with the x-ray source based at 70 kVp and 500 µA and an exposure time of 180 s (0.5 s per projection). A Feldkamp reconstruction algorithm was applied [21 ]. A Medical Imaging Data Examiner (AMIDE) software (http://amide.sourceforge.net ) was used to view PET and CT scans. Images are displayed as 25 mm (coronal) or 2 mm (transverse) maximum intensity projections (MIP). Following the final PET/CT scan, ex vivo biodistribution was performed. Organs were removed, weighed, and counted in a Wizard 3” automatic gamma counter (Perkin Elmer) and the decay-corrected percent injected dose/gram (% ID/g) for each organ was calculated.
Wizard 3 automatic gamma counter
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2 protocols using wizard 3 automatic gamma counter
1
Multimodal Imaging of Zr-89 Labeled Antibody
2
Quantifying Fetal FDG Uptake in Mice
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18F-FDG was obtained from the UCLA Department of Nuclear Medicine. Warmed pregnant mice or pups were injected intravenously or intraperitoneally, respectively, with ~90 microCi (~3.33 MBq) of 18F-FDG. After 2 hr, the mice were sacrificed. Preliminary experiments suggested that 2 hr was sufficiently long for 18F-FDG to reach maximum accumulation in each organ and embryo. Fetal or neonatal hearts were separated from the other tissue (carcass), and the mass and radioactivity in both the hearts and the carcasses were measured using a standard balance and a Wizard 3’ automatic gamma counter (Perkin Elmer), respectively. The radioactivity levels in the pup carcasses were higher than the detection limit of the gamma counter, so the expected gamma counter values for the pup carcasses were calculated on the basis of the decay-corrected injected dose of 18F-FDG and known conversion values between microCi and CPM on the gamma counter. To calculate the ‘Normalized FDG accumulation’, radioactive accumulation in each heart was divided by heart weight and then further divided by the total radioactivity in each embryo or pup. This last normalization is to account for differences in 18F-FDG injected dose and accessibility to the embryos and pups. Averages and standard errors of the mean (SEM) were calculated, and the values were normalized such that E10.5 embryo FDG accumulation was set to 100.
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