Jnm ecs 400 mhz

Sephadex LH-20 (Merck KGaA, Darmstadt, Germany), silica gel 60 (Merck KGaA) and Geduran Si 60 (Merck KGaA) were used for column chromatography. TLC plates (Silica Kiesel 60 F254) were from Merck KGaA. Jasco V-530 ultraviolet spectrophotometer (Jasco International Co., Ltd, Tokyo, Japan) was used to measure UV spectra. IR spectra were obtained on an FT-IR-4100 Jasco spectrophotometer (Jasco). Optical rotations were achieved by a Jasco P-2000 digital polarimeter (Jasco). NMR spectra were obtained by JEOL JNM ECS 400 MHz. Electrospray ionization mass spectrometry (ESIMS) data were collected on a Waters micromass ZQ mass spectrometer (Waters Corporation, Milford, MA, USA). High-resolution ESIMS data was accomplished by a Bruker APEX II spectrometer (FT-ICR/MS, FTMS) (Bruker Daltonics Inc., Billerica, MA, USA). Dulbecco’s modified Eagle’s medium (high glucose) powder (DMEM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), p-nitrophenyl-N-acetyl-d-glucosaminide (p-NAG), penicillin and streptomycin, dexamethasone, calcium ionophore A23187, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT, USA). Mouse anti-DNP IgE antibody was a generous gift from Dr. Daniel H. Conrad (Virginia Commonwealth University, Richmond, VA, USA).

Sephadex (GE healthcare) LH-20 and silica gel 60 (Merck KGaA, Darmstadt, Germany) were used for open-column chromatography (CC). Luna C₁₈, phenyl-hexyl (5 μm, 250 × 10 mm, Phenomenex, Torrance, CA, USA) semi-preparative columns were used for high-performance liquid chromatography (HPLC). HPLC instrument used a Shimadzu LC-10AT pump with an SPD-20A UV-Vis detector. The UV spectra were obtained by using a Jasco UV-530 ultraviolet spectrophotometer (Jasco, Tokyo, Japan), whereas the IR spectra were obtained on a Jasco FT-IR-4600 spectrophotometer (Jasco, Tokyo, Japan). Optical rotations were measured with a Jasco P-1020 digital polarimeter (Jasco, Tokyo, Japan). NMR spectra were obtained using JEOL JNM ECS 400 MHz (JEOL, Tokyo, Japan), Varian 600 MHz NMR (Varian, Palo Alto, CA, USA), and Bruker AVIIIHD700X 700 MHz spectrometers (Bruker, Bremen, Germany). ESI–MS data were collected on a VG Biotech Quattro 5022 mass spectrometer (VG Biotech, Altrincham, UK). High-resolution ESI–MS data were obtained with a Bruker APEX II spectrometer (Bruker, Bremen, Germany). Circular dichroism spectra were recorded on a JASCO J-810 spectrophotometer (Jasco, Tokyo, Japan).