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Ly6g apc clone 1a8

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Ly6G-APC (clone 1A8) is a fluorochrome-conjugated antibody that specifically binds to the Ly6G antigen. Ly6G is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the surface of neutrophils. This product can be used for the identification and enumeration of neutrophils in flow cytometry applications.

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Lab products found in correlation

Ly6c percpcy5.5 clone al 21, by BD (2 mentions) Cd11b pe clone m1 70, by BioLegend (1 mentions) Cd3 pe clone 145 2c11, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by FlowJo (1 mentions) Fc block, by Thermo Fisher Scientific (1 mentions) Fcs express, by De Novo Software (1 mentions) Cd11b efluor 450 clone m1 70, by Thermo Fisher Scientific (1 mentions) Zombie aqua fixable viability kit, by BioLegend (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Lsr 2, by BD (1 mentions) Collagenase d, by Roche (1 mentions) Rpmi 1640 medium, by Biowest (1 mentions) Siglec f pe clone e50 2440, by BD (1 mentions) F4 80 bv421 clone bm8, by BioLegend (1 mentions) Cd8 fitc clone 53 6, by BioLegend (1 mentions) Cd4 apc cy7 clone rm4 5, by BioLegend (1 mentions) Cd3 fitc clone 145 2c11, by BioLegend (1 mentions) Cd49b apc clone dx5, by BioLegend (1 mentions) Cd11b pe cy7 clone m1 70, by BioLegend (1 mentions)

3 protocols using ly6g apc clone 1a8

1

Comprehensive Immune Cell Profiling in Lung Tissue

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Lung cells (1 × 106 to 2 × 106) were stained for surface antigens for 30 min at 4°C. Stained cells were washed and then fixed overnight in PBS containing 2% paraformaldehyde. The following antibodies were used: CD3-PE (clone 145-2C11; eBioscience), CD4-PB (clone RM4-5; eBioscience), CD8-FITC (clone 5H10-1; BioLegend), CD44-PerCPCy5.5 (clone 1M7; eBioscience), CD62L-PECy7 (clone MEL-14; BioLegend), CD19-APC (clone eBio1D3; eBioscience), Ly6G-APC (clone 1A8; BioLegend), Ly6C-PerCPCy5.5 (clone AL-21; BD Pharmingen), MHC II-FITC (clone AMS-32.1; BD Pharmingen), CD11b-PE (clone M1/70; BioLegend), and CD11c-PB (clone N418; BioLegend). Samples were run on an LSRII flow cytometer with Diva software, and the data were analyzed using FlowJo version 10.1.r7 software. The total number of cells in each gate was calculated using the total number of cells determined by the Countess automated cell counter. The gating strategy used is shown in Fig. S2 in the supplemental material.
Bhatt K., Machado H., Osório N.S., Sousa J., Cardoso F., Magalhães C., Chen B., Chen M., Kim J., Singh A., Ferreira C.M., Castro A.G., Torrado E., Jacobs WR J.r., Bhatt A, & Saraiva M. (2018). A Nonribosomal Peptide Synthase Gene Driving Virulence in Mycobacterium tuberculosis. mSphere, 3(5), e00352-18.
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2

Skin Sample Analysis Protocol

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0.5‐cm2 skin samples (from the central and lateral skin) were incubated with 2.5 mg/mL of Collagenase D (Roche Diagnostics) solution. Single‐cell suspensions from the skin were obtained by mashing the samples through 40‐μm cell strainers in RPMI1640 medium (Biowest) supplemented with 2% FBS (Gibco). The cells were stained for viability assessment (Zombie Aqua Fixable Viability Kit; BioLegend) and then blocked with anti‐CD16/CD32 antibodies (Fc block; eBioscience) followed by staining with directly conjugated antibodies: CD45.2‐APC/Cy7 (clone 104, BioLegend), CD11b‐eFluor450 (clone M1/70, eBioscience), Ly6G‐APC (clone 1A8, BioLegend) and CD3‐Alexa Fluor 594 (clone 17A2, BioLegend). Data were acquired on a BD LSRII (BD Biosciences). Singlets were selected based on FCS‐A vs FCS‐H. Dead cells were routinely excluded from the analysis. Analyses were performed with FCS Express (De Novo Software).
Kwiecinska P., Grygier B., Morytko A., Sanecka‐Duin A., Majchrzak‐Gorecka M., Kwitniewski M., Kapinska‐Mrowiecka M., Porebski G, & Cichy J. (2022). Secretory leukocyte protease inhibitor regulates nerve reflex‐mediated skin barrier function in psoriasis. Journal of the European Academy of Dermatology and Venereology, 36(8), 1266-1274.
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3

Comprehensive Immune Cell Profiling

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CD3-FITC clone 145-2C11, CD4-APC-Cy7 clone RM4-5, CD8-FITC clone 53-6.7, CD45-AF700 clone 104, CD11b-PE-Cy7 clone M1/70, CD11c-BV450 clone N418, CD19-PerCP-Cy5.5 clone 6D5, CD49b-APC clone DX5, F4/80-BV421 clone BM8, IL-17A-PE-Cy7 clone TC11-18H10.1, Ly6G-APC clone 1A8, and TCRγδ-BV421 clone GL3, and were purchased from Biolegend (San Diego, CA). Ly6C-PerCP-Cy5.5 clone AL-21, RORγt-BV650 clone Q31-378, and Siglec F-PE clone E50-2440 were purchased from BD Biosciences (San Jose, CA). Foxp3-PE clone 150D/E4 was purchased from ThermoFisher Scientific (Waltham, MA).
Denny J.E., Powers J.B., Castro H.F., Zhang J., Joshi-Barve S., Campagna S.R, & Schmidt N.W. (2019). Differential Sensitivity to Plasmodium yoelii Infection in C57BL/6 Mice Impacts Gut-Liver Axis Homeostasis. Scientific Reports, 9, 3472.
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