7600 automated biochemistry analyzer

Manufactured by Hitachi

Sourced in Japan

The Hitachi 7600 automated biochemistry analyzer is a laboratory instrument designed for the analysis of biochemical samples. It is capable of performing a variety of routine clinical chemistry tests, such as measuring the levels of enzymes, proteins, and other analytes in biological fluids. The instrument utilizes advanced technology to automate the analytical process, providing efficient and accurate results.

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Lab products found in correlation

Bovine elisa kits, by Nanjing Jiancheng (1 mentions) Vacutainer, by BD (1 mentions) C57bl 6, by Beijing Huafukang Biotechnology (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Inverted microscope, by Olympus (1 mentions) Niox mino, by NIOX Group (1 mentions) Xe 2100 fully automatic hematology analyzer, by Sysmex (1 mentions)

6 protocols using 7600 automated biochemistry analyzer

Blood Metabolite and Antioxidant Analysis in Cows

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Blood was collected from the tail vein of cows using 10 mL blood collection heparin-coated tubes (Vacutainer; Becton Dickinson, Franklin Lakes, NanJing) before morning feeding every 14 days. In each group, the five animals that had been used for fecal sampling were used for blood sampling. Blood samples were centrifuged at 3500× g at 4 °C for 15 min to obtain plasma and then stored at −20 °C for further analysis.
All of the blood plasma samples were analyzed by a Hitachi 7600 automated biochemistry analyzer (Hitachi Co., Tokyo, Japan). Glucose, insulin, glucagon, β-hydroxybutyric acid (BHBA), triglyceride (TG), glutamic-pyruvic transaminase (GPT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total protein (TP) were measured using blood colorimetric commercial kits (DiaSys Diagnostics Systems GmbH, Frankfurt, Germany). Albumin (ALB), globulin (GLB), the total antioxidant capacity (T-AOC), malonaldehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), immune globulin A (IgA), immune globulin G (IgG), immune globulin M (IgM), and anti-tumor necrosis factor-α (TNF-α) were measured using bovine ELISA kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China).

Hao Y., Huang S., Si J., Zhang J., Gaowa N., Sun X., Lv J., Liu G., He Y., Wang W., Wang Y, & Li S. (2020). Effects of Paper Mulberry Silage on the Milk Production, Apparent Digestibility, Antioxidant Capacity, and Fecal Bacteria Composition in Holstein Dairy Cows. Animals : an Open Access Journal from MDPI, 10(7), 1152.

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Effects of GDC0941 and MK2206 on Atherosclerosis

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ApoE knockout male mice (6‐week old) with C57BL/6 background were obtained from HFK Bioscience (Beijing, China) and maintained on a high-fat diet (34% fat/1.25% cholesterol, Trophic Animal Feed High-Tech Co., Ltd., Nantong, China). All experimental procedures were approved by the Institutional Animal Care Committee of Dalian Medical University (Dalian, China). The mice (n=24) were divided into three groups randomly (n=8 per group): high-fat diet (HFD), GDC0941 + high-fat diet (GDC0941 + HFD) and MK2206 + high-fat diet (MK2206 + HFD). The mice in GDC0941 + HFD and MK2206 + HFD groups were administered daily by oral gavage. After 12 weeks on a high-fat diet, the mice were euthanized using 1% sodium pentobarbital (50 mg/kg; Sigma-Aldrich) and tissue samples were collected for further analyses. Histological images of aortic sinus lesions were obtained by hematoxylin and eosin (HE) and oil red O staining. Total cholesterol (TC), triglycerides (TG), low‐density lipid cholesterol (LDL‐C), and high‐density lipid cholesterol (HDL‐C) in serum samples were detected using a Hitachi 7600 automated biochemistry analyzer. NLRP3 and IL-1β were determined by immunohistochemistry and photographed using an inverted microscope (Olympus).

Liu Z., Li J., Lin S., Wu Y., He D, & Qu P. (2021). PI3K regulates the activation of NLRP3 inflammasome in atherosclerosis through part-dependent AKT signaling pathway. Experimental Animals, 70(4), 488-497.

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Impact of Intensive Lipid Lowering on Carotid Atherosclerosis in Stroke Patients

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We retrospectively assessed data that was prospectively collected on 106 adult stroke patients, whose etiology was thought to be carotid atherosclerosis [10 (link)], at Xuanwu hospital stroke center, from January 2013 to December 2014. We excluded pregnant patients or patients have past medical histories of intracranial hemorrhage, hepatic dysfunctions, kidney dysfunctions, and familial hypercholesterolemia.
Patients were assessed by B-mode ultrasound at baseline before therapy and 12 months after treatment to characterize the common carotid atherosclerosis plaques. The parameters of carotid ultrasound include length and thickness on both sides of the common carotid artery at carotid bulbs. All the patients were treated with various types and dose of statins according to the secondary stroke prevention guideline [4 (link)]. We divided the patients into ILLT (<70 mg/dL) group and CLLT (70–120 mg/dL) group according to their end-point LDL-c levels at 12 months post-therapy. Blood examinations for lipid levels, homocysteine (HCY) levels and glycemic levels were performed at one year after lipid-lowering therapy. Serum blood biochemistry tests were tested using Hitachi 7600 automated biochemistry analyzer (Hitachi, Ltd, Japan).
This study was approved and conducted in compliance with Institutional Review Board (IRB) of Xuanwu Hospital, Capital Medical University.

Zhang Q., Liu S., Liu Y., Hua Y., Song H., Ren Y., Song Y., Liu R., Feng W., Ovbiagele B., Ding J, & Ji X. (2017). Achieving low density lipoprotein-cholesterol < 70 mg/dL may be associated with a trend of reduced progression of carotid artery atherosclerosis in ischemic stroke patients. Journal of the neurological sciences, 378, 26-29.

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Comprehensive Serum Biomarker Analysis

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At the end of the trial, 10 mL of blood was collected from the jugular vein after fasting for 12 h. Serum samples were centrifuged at 3000 rpm for 10 min and stored at −20 °C for analysis.
Total protein (TP), albumin (ALB), globulin (GLB), triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), γ-glutamyl transpeptidase (γ-GT), serum uric acid (UA), serum urea nitrogen (UREA), serum calcium (Ca), serum phosphorus (P), serum ammonia, immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) were measured by Hitachi 7600 automated biochemistry analyzer (Hitachi Co., Tokyo, Japan). Serum superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) were detected using a commercial ELISA kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China). And serum anti-tumor necrosis factor-α (TNF-α) was determined using a Multiskan MK₃ microplate tester (Thermo Fisher Scientific, Waltham, MA, USA).

Chen Y., Dong B., Qu H., Cheng J., Feng Y., Liu L, & Ma Q. (2023). Evaluating the Effects of Replacing Alfalfa with Broussonetia papyrifera Branch/Leaf Powder on Growth and Serum Indicators in Dezhou Donkeys. Animals : an Open Access Journal from MDPI, 14(1), 123.

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Multidimensional Asthma Assessment Protocol

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Multidimensional assessments, including sociodemographic characteristics, asthma duration, smoking history, allergen detection results, comorbidities, medication use and acute asthma exacerbation, were performed for all included patients. Asthma control was assessed using the Asthma Control Questionnaire (ACQ) (12 (link)), the Mini Asthma Quality of Life Questionnaire (Mini-AQLQ) (13 (link)), and the Asthma Control Test (ACT) (14 (link)).
The participants also underwent spirometry, FeNO tests, routine blood tests and serum total IgE detection. The FeNO test was performed using a FeNO analyzer (NIOX MINO, Aerocrine AB, Sweden). After the FeNO test, pulmonary function and bronchial dilation tests were performed using spirometry (Master Screen-PFT, Jaeger, Germany). Routine blood tests were performed with a hematology analyzer (Sysmex XE-2100 Fully Automatic Hematology Analyzer, Sysmex, Japan). Serum total IgE was measured by immunoassay (Hitachi 7600 automated biochemistry analyzer, Hitachi, Japan), with a minimum detectable level of IgE of 1.0 IU/mL.

Mao R., Jiang Z., Min Z., Wang G., Xie M., Gao P., Zhu L., Li H, & Chen Z. (2023). Peripheral neutrophils and oxidative stress-associated molecules for predicting the severity of asthma: a cross-sectional study based on multidimensional assessment. Frontiers in Medicine, 10, 1240253.

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Urinary Biomarker Measurement Protocol

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Urinary sodium (Na) and potassium (K) in the urine were detected by the ion-selective electrode method, and urinary creatinine (Cr) was assayed by the sarcosine oxidase method. These assays were performed on the HITACHI 7600 automated biochemistry analyzer(Hitachi Co., Tokyo, Japan). The standard solution for machine validation was tested for every 100 urine samples. Quality-control measures of the test included calculating the covariance of the standard solution centration and checking the difference between the concentration of the normal urine samples and the blind urine samples. For this analysis, the following urine samples were excluded: (1) the self-reported 24 h missed urine volume was over 20% of the total volume; (2) the total 24 h urine volume was less than 500 mL; (3) the 24 h urinary creatinine excretion (24UCrV) was < 4 mmol or >25 mmol in women or <6 mmol or >30 mmol in men [27 (link)]; (4) the 24 h urine volume was more than 3 standard deviations of the population mean [28 (link)]; (5) the spot urinary creatinine concentration (spot _Cr) was more than 3 standard deviations of the population mean.

Wu B., Yang H., Ren X., Qi Z., Tang S., Yin X., Huang L., Tian M., Wu Y., Feng X, & Li Z. (2022). A Method for Estimating 24 h Urinary Sodium and Potassium Excretion by Spot Urine Specimen in Stroke Patients. Nutrients, 14(19), 4105.

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