Hep293tt

The rhabdomyosarcoma (SJCRH30), Ewing sarcoma (A-673), and medulloblastoma (D283) cell lines were purchased from the ATCC (Manassas, VA, USA). The hepatoblastoma cell line (Hep293TT) was obtained directly from Dr. Gail Tomlinson.¹⁸ The SJCRH30, A-673, and Hep293TT cells were grown in RPMI-1640 medium (Corning) with 5% fetal bovine serum (Corning) and 0.5% gentamycin (Gibco), while the D283 cells were grown in IMEM (Gibco) with 5% fetal bovine serum and 0.3% gentamycin. Cells were grown at 37 °C in a humidified incubator with 5% CO₂. Cells were used within four months of retrieval from liquid nitrogen. The SJCRH30, D283, and A-673 cells lines were validated by STR profiling (Genetica).

The Ewing sarcoma (A-673, SK-ES-1), rhabdomyosarcoma (SJCRH30), medulloblastoma (D283, D341, DAOY), and breast cancer (HCC1806) cell lines were purchased from the American Type Culture Collection, (Manassas, VA, USA). The hepatoblastoma cell line (Hep293TT) was obtained directly from Dr. Gail Tomlinson.^{23 (link)} A-673, SK-ES-1, SJCRH30, HCC1806, and Hep293TT cells were grown in RPMI-1640 medium (Corning, Corning, NY, USA) with 5% fetal bovine serum (Corning) and 50 μg/mL gentamicin (Gibco/Thermo Fisher Scientific, Waltham, MA, USA). The D283, D341, and DAOY cells were grown in IMEM (Gibco) with 5% fetal bovine serum and 25 μg/mL gentamicin. Cells were grown at 37 °C with 5% CO₂ in humidified incubators. The cells were used within four months of retrieval from liquid nitrogen. The A-673, SJCRH30, and D283 cells lines were validated by STR profiling (Genetica DNA Laboratories, Burlington, NC, USA).