Serum parameters including total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol levels were measured with the commercial kits (A111-2, A112-2, and A113-2; Jiancheng). Serum chemistry tests including alanine aminotransferase, aspartate aminotransferase, creatine kinase, lactate dehydrogenase, urea nitrogen, creatinine, uric acid, albumin, and total protein were performed with the commercial kits (MAK052, MAK055, MAK116, MAK066, MAK006, MAK080, MAK077; 09753 and 71285-M; Sigma–Aldrich). Triglyceride was extracted using 5% NP-40 solution in liver and heated at 90 °C for twice. Then it was cooled down to room temperature and centrifuged at 12,000 rpm for 2 min to collect transparent supernatant. The serum and hepatic levels of triglyceride were measured using TG kit (K952; BioVision).
A113 2
Manufactured by Nanjing Jiancheng
Sourced in China
The A113-2 is a laboratory equipment designed for scientific research and analysis. It is a compact and versatile device that serves as a core component in various laboratory settings. The A113-2 offers reliable performance and consistent results, making it a valuable tool for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
A111 2, by Nanjing Jiancheng (2 mentions)
Mak066, by Merck Group (1 mentions)
Mak052, by Merck Group (1 mentions)
Mak055, by Merck Group (1 mentions)
Mak077, by Merck Group (1 mentions)
Mak080, by Merck Group (1 mentions)
Mak006, by Merck Group (1 mentions)
Mak116, by Merck Group (1 mentions)
C009 2, by Nanjing Jiancheng (1 mentions)
A110 2, by Nanjing Jiancheng (1 mentions)
Ezrmi 13k, by Merck Group (1 mentions)
2 protocols using a113 2
1
Serum and Hepatic Lipid Profiling
2
Metabolic Assessment in Fasted Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were fasted for 16 h before the tests. For the glucose tolerance tests (GTT), 2 g/kg glucose was injected intraperitoneally and plasma glucose levels were measured before the injection and at 15, 30, 60, 90, 120 and 150 min after the injection. For insulin tolerance tests (ITT), 0.75 U/kg insulin was given intraperitoneally and glucose levels in plasma were also determined before the injection and at 15, 30, 60, 90, 120 and 150 min after the injection. Fasting insulin levels was detected by an ELISA kit (EZRMI-13K, Millipore, Billerica, MA). Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as fasting insulin concentration (mU/L) × fasting glucose concentration (mmol/L)/ 22.5. Hepatic and plasma triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and alanine aminotransferase (ALT) were measured by enzymatic assay kits (A110-2, A111-2, A113-2, C009-2 Nanjing Jiancheng Bioengineering Institute, China). Serum levels of calcium and phosphate was determined by spectrophotometry (BC1655, BC0720, Beijing Solarbio Science & Technology Co. Ltd, China).
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