Proclin 950

Manufactured by Merck Group

Sourced in United States

Proclin 950 is a preservative used in laboratory applications. It is a biocidal agent designed to protect aqueous formulations from microbial contamination.

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Lab products found in correlation

Donkey serum, by Merck Group (1 mentions) Ab82527, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Dojindo Laboratories (1 mentions) Sc 28319, by Santa Cruz Biotechnology (1 mentions) Probumin, by Merck Group (1 mentions) Anti rabbit or anti mouse igg, by Merck Group (1 mentions)

2 protocols using proclin 950

Isolation of Type A Spermatogonia

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Three days or one week later, testes were dissociated as described in the ‘Testis dissociation’ section to prepare the following four samples: 1. Three testes from three days treatment, 2. Nine testes from three days treatment, 3. Three testes from one week treatment 4. Nine testes from one week treatment. Dissociated testicular cells were permeabilized with 70% EtOH on ice for 30 min, washed twice with DPBS containing 5% Probumin (Merck Millipore), 0.1% Donkey Serum (Sigma), and 0.01% of Proclin 950 (Sigma), incubated with anti-Gcna1 (Abcam, ab82527, 1:200) and Plzf (Santa Cruz, sc-28319, 1:50) antibodies at 4°C overnight, washed twice, and stained with Alexa Fluor 555 (for Gcna1)- and Alexa Fluor 488 (for Plzf)- conjugated secondary antibodies (Invitrogen, 1:400) and DAPI (Dojindo, 1:1,000). The Gcna1⁺/Plzf⁺ Type A Spermatogonia were gated and sorted by FACS Aria IIIu, following the same procedure as sperm sorting for subsequent gDNA extraction.

Noguchi Y., Onodera Y., Miyamoto T., Maruoka M., Kosako H, & Suzuki J. (2024). In vivo CRISPR screening directly targeting testicular cells. Cell Genomics, 4(3), 100510.

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Immunohistochemistry for CRP, PTX3 and SAP

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Tissue from the 13 autopsy cases underwent a citrate antigen retrieval for 20 min at 97 °C. Mouse monoclonal antibody with CRP or PTX 3 or a rabbit polyclonal antibody with SAP (all Sigma-Aldrich, St. Louis, MO, USA) were diluted 1/300 in Tris-buffered saline, and added along with surfactant, protein stabilizer, and 0.35 ProClin 950 (Sigma-Aldrich) for 30 min. The secondary antibodies were anti-mouse or anti-rabbit IgG (Sigma-Aldrich) and diluted in Tris-buffered saline containing 10% animal serum and 0.09% ProClin 950 from Leica’s Refine Detection Kit (Leica Biosystems Newcastle Ltd., Newcastle Upon Tyne, UK). The secondary antibody was tagged with horseradish peroxidase, either brown or red. The brown antibody reactions were difficult to interpret in some cases, particularly the CRP antibody, because of lipofuchsin present in the tissue (it appears brown on light microscopy). Therefore, with CRP antibodies we measured the binding of antibodies to fungi with the red reaction only. Human tissue that tested positive for CRP, PTX3, and SAP were kidney, prostate and kidney, respectively.

Golconda U., Sobonya R.E, & Klotz S.A. (2018). Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis?. Journal of Fungi, 4(3), 111.

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