The following antibodies were used for Western blot (WB) and/or immunofluorescence (IF): NUP133 (sc-376763, Santa Cruz Biotechnology Inc., Dallas, TX, USA, WB 1:2000, IF 1:300), NUP107 (SAB2702098, Merck, WB 1:1000, IF 1:200), nuclear pore complex proteins mAb414 (ab24609, Abcam, Cambridge, UK, WB 1:1000, IF 1:200), beta-Actin (4970S, Cell Signaling Technology Inc., Danvers, MA, USA, IF 1:400), VCL (HPA063777, Atlas Antibodies AB, Bromma, Sweden, WB 1:1000), TUBA (T9026, Merck, WB 1:5000), FLAG M2 (F3165, Merck, WB 1:5000), PXN (610051, BD Biosciences, Franklin Lakes, NJ, USA, NU, 1:300), COL18A1 (ab275746, Abcam, WV 1:1000), PPME1 (sc-25278, Santa Cruz Biotechnology, WB 1:2000), Hoechst 33342, trihydrochloride, trihydrate (H3570, Thermo Fisher Scientific, IF 1:1000), and Alexa Fluor phalloidin 488/555 (Thermo Fisher Scientific, IF 1:200–1:750). For IF Alexa Fluor conjugated anti-mouse or anti-rabbit secondary antibodies (A-31572, A-21127, A21245, A31570, A28175, Thermo Fisher Scientific, IF 1:500) and WB HRP-linked, anti-mouse (goat anti-mouse immunoglobulins/HRP, P0447, Dako, WB 1:10,000) or anti-rabbit (goat anti-rabbit IgG, HRP-linked antibody, #7074, cell signaling, WB 1:1000) secondary antibodies were used.
Ab275746
Manufactured by Abcam
Sourced in United States
Ab275746 is a lab equipment product manufactured by Abcam. It is a device designed for use in research and scientific applications, but its core function is not detailed in the available information.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
A31572, by Thermo Fisher Scientific (1 mentions)
A 21245, by Thermo Fisher Scientific (1 mentions)
A21127, by Thermo Fisher Scientific (1 mentions)
Flag m2, by Merck Group (1 mentions)
H3570, by Thermo Fisher Scientific (1 mentions)
A31570, by Thermo Fisher Scientific (1 mentions)
T9026, by Merck Group (1 mentions)
A28175, by Thermo Fisher Scientific (1 mentions)
P0447, by Agilent Technologies (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Ab24609, by Abcam (1 mentions)
Nup133, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene di uoride membrane, by Merck Group (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
3 protocols using ab275746
1
Comprehensive Antibody Characterization for Cell Imaging
2
Western Blot Analysis of COL18A1
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Equal amounts of protein samples (30 μg) were extracted with RIPA lysis buffer (Sigma, USA) and uploaded into 10% sodium dodecyl sulfate polyacrylamide gels for protein separation. The proteins were transferred to polyvinylidene di uoride membranes (Millipore, USA) and then sealing the membranes with 5% skimmed milk, the membranes were incubated with primary anti-COL18A1 (1:1000, Abcam, ab275746) and anti-rabbit secondary antibody (1:5000, Thermo Fisher). Immune binding was detected using an ECL detection system (Shanghai Peiqing Technology Co., Ltd., China).
3
Immunohistochemical Analysis of COL18A1 in PTC
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Human PTC tissues were collected from patients who underwent thyroidectomy at The Second Hospital of Tianjin Medical University. The study was approved by the Ethics Committee of The Second Hospital of Tianjin Medical University and written informed consent was obtained from these patients. The clinicopathological data of the patients are listed in Table 2, and a total of 119 patients were included in the study. 119 tumor tissue samples and 15 normal paracancerous tissue samples were selected for immunohistochemical staining. 4% paraformaldehyde was xed, para n embedded, sectioned into 5 μm thick sections and dewaxed and dehydrated. After antigen repair and endogenous peroxidase disruption, sections were incubated with Anti-COL18A1(Abcam, ab275746) antibody overnight, followed by incubation with secondary antibody. Sections were treated with horseradish peroxidase and 3,3'diaminobenzidine and then stained with hematoxylin. Light microscopy was used to capture images and immunohistochemical scoring using the 12-point scale [19] .
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