Log In Sign up
The largest database of trusted experimental protocols

Western blot kit

Manufactured by Vector Laboratories
Visit Supplier

The Western blot kit is a laboratory tool used for the detection and analysis of specific proteins in a complex sample. It consists of a set of reagents and materials necessary to perform the Western blot technique, which involves the separation of proteins by size, transfer to a membrane, and subsequent detection using specific antibodies.

Automatically generated - may contain errors

Lab products found in correlation

Streptavidin horseradish peroxidase, by Vector Laboratories (2 mentions) Pvdf membrane, by Vector Laboratories (2 mentions) Lens culinaris agglutinin lca, by Vector Laboratories (2 mentions) Pvdf membrane, by Merck Group (2 mentions)

2 protocols using western blot kit

1

Lens Culinaris Agglutinin Glycoprotein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The CRC cells were lysed and separated via 10% SDS-PAGE and transferred to polyvinylidene di uoride (PVDF) membranes (Millipore, Bedford, MA, USA). Then the PVDF membranes were incubated with the biotinylated Lens Culinaris Agglutinin (LCA) (Vector Laboratories, Burlingame, CA, USA) for 1h at room temperature. The membranes were subsequently incubated with horseradish peroxidase streptavidin (Vector Laboratories) for 30 min and detected with an ECL Western blot kit.
Qi Y., Shan Y., Li S., Huang Y., Guo Y., Huang T., Zhao X, & Jia L. (2022). LncRNA LEF1-AS1/LEF1/FUT8 Axis Mediates Colorectal Cancer Progression by Regulating α1, 6-Fucosylationvia Wnt/β-Catenin Pathway. Digestive diseases and sciences, 67(6).
+ Open protocol
+ Expand
2

Lens Culinaris Agglutinin Glycoprotein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The CRC cells were lysed and separated via 10% SDS-PAGE and transferred to polyvinylidene di uoride (PVDF) membranes (Millipore, Bedford, MA, USA). Then the PVDF membranes were incubated with the biotinylated Lens Culinaris Agglutinin (LCA) (Vector Laboratories, Burlingame, CA, USA) for 1h at room temperature. The membranes were subsequently incubated with horseradish peroxidase streptavidin (Vector Laboratories) for 30 min and detected with an ECL Western blot kit.
Qi Y., Shan Y., Li S., Huang Y., Guo Y., Huang T., & Li J. (2020). LncRNA LEF1-AS1/LEF1/FUT8 Axis Mediates Colorectal Cancer Progression by Regulating α1, 6-Fucosylation via Wnt/β-Catenin Pathway.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!