Ab201227

mAbs and their sources included mouse anti-TYRP1 (TA99, also known as Mel-5; American Type Culture Collection) and mouse anti-Myc for dIFM (9E10; American Type Culture Collection), mouse anti-Pldn (clone 2G6; a generous gift from Esteban Dell’Angelica, University of California, Los Angeles, CA; Ghiani et al., 2010 (link)), mouse anti–AP-3δ (clone SA4; Developmental Studies Hybridoma Bank), rabbit anti-AP3M1 (ab201227; Abcam), mouse anti–γ-tubulin (GTU88; Sigma-Aldrich), mouse anti-VAMP7 (clone 158.2; Synaptic Systems), mouse anti-GFP (NB600-597, Novus Biologicals; or clones 7.1 and 13.1, Roche), and mouse anti-clathrin heavy chain (clone 23; BD Biosciences). Polyclonal antibodies included rabbit anti-STX13 (Prekeris et al., 1998 (link)), as previously described (Setty et al., 2007 (link)); rabbit anti-VAMP7 TG50, a kind gift from Thierry Galli (Verraes et al., 2018 (link)); rabbit αPep7h-msm to the cytoplasmic domain of TYR (Berson et al., 2000 (link)); rabbit anti-dysbindin (Starcevic and Dell’Angelica, 2004 (link)), a kind gift from Esteban Dell’Angelica; and sheep anti-TGN46 (Serotec). Species-specific secondary antibodies from donkey and conjugated to Alexa Fluor 488, Cy3, Alexa Fluor 594, and Alexa Fluor 647 were used for dIFM or to IRDye-790CW, IRDye-680LT, or HRP for immunoblotting (Jackson ImmunoResearch Laboratories, Inc.).

Primary monoclonal antibodies used and their sources (indicated in parentheses) were: mouse anti-TYRP1 (TA99/Mel-5; American Type Culture Collection); mouse anti-γ-Tubulin (GTU-88; Sigma-Aldrich); mouse anti-γ-adaptin (610385; BD); mouse anti-GFP (clones 7.1 and 13.1; Roche); rabbit anti-AP3M1 (ab201227; Abcam); rat anti–mouse LAMP2 (GL2A7; Abcam); rat anti-mouse TfR (CD71; BD 553264); and rat anti-HA (3F10; Roche 11867423001). Primary polyclonal antibodies used and their sources were: rabbit anti-STX13 (a kind gift of Rytis Prekeris, University of Colorado, Denver, CO; Prekeris et al., 1998 (link)); rabbit anti-pallidin (a kind gift of Juan Bonifacino, National Institute of Child Health and Human Development, Bethesda, MD; Moriyama and Bonifacino, 2002 (link)); rabbit anti-PI4KIIIβ (13247-1-AP; Proteintech); rabbit anti-PI4KIIα and anti-PI4KIIβ (kind gifts of Pietro De Camilli, Yale University, New Haven, CT; Guo et al., 2003 (link)); and rabbit anti-GFP (PABG1; Chromotek); Species- and/or mouse isotype–specific secondary antibodies from donkey or goat conjugated to Alexa Fluor 488, Alexa Fluor 594, or Alexa Fluor 640 used for IFM or to Alexa Fluor 680 or Alexa Fluor 790 for immunoblots were obtained from Jackson ImmunoResearch Laboratories.